Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock
Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock
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DOI：
10.1016/s1532-0456(01)00165-x
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发表时间：
2001-03-01
影响因子：
3.9
通讯作者：
Nemcsók, J
中科院分区：
文献类型：
--
作者：
Hermesz, E;Abrahám, M;Nemcsók, J
采用RT-PCR方法从鲤鱼（Cyprinus carpio）中分离到两种金属硫蛋白（MT-1和MT-2）的cDNA异构体。cDNA序列分析显示编码区之间存在两个氨基酸差异，3 '端非翻译末端明显不同。选择基因特异性引物并用于RT-PCR反应中以测量基础MT-1和MT-2 mRNA水平，并在体内研究期间跟踪MT基因在不同组织中的诱导剂特异性表达。在大脑和肌肉中，两种MT mRNA的未诱导水平相似。在肾脏和肝脏中，MT-I基因产物占主导地位，而在心脏中，两种基因的相对表达水平相反。MT-1和MT-2 mRNA水平均随镉浓度的增加而增加，且呈时间和剂量依赖性。MT-2的表达，然而，更响应于高浓度的镉。在平行的诱导的MT镉，我们遵循的积累，这种金属在肾脏和肝脏。虽然镉水平始终较高，在治疗过程中的肾脏，积累率较高的肝脏。冷胁迫导致MT-1的诱导显著高于MT-2，而热激对这两个基因的表达没有影响。(C)2001 Elsevier Science Inc. All rights reserved.
Two metallothionein cDNA isoforms (MT-1 and MT-2) were isolated from carp (Cyprinus carpio) by RT-PCR. Sequence analysis of the cDNAs revealed two amino acid differences between the coding regions and markedly different 3'-untranslated ends. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal MT-I and MT-2 mRNA levels and to follow the inducer-specific expression of MT genes in different tissues during in vivo studies. In the brain and muscle, the uninduced levels of the two MT mRNAs are similar. In the kidney and liver, the MT-I gene product predominated, while in the heart the relative expression levels of the two genes were opposite. Both the MT-I and MT-2 mRNA levels increased with Cd concentration in a time- and dose-dependent manner. The expression of MT-2, however, was more responsive to a high Cd concentration. In parallel with the induction of the MTs by Cd, we followed the accumulation of this metal in the kidney and liver. Although the Cd level was always higher in the kidney during treatment, the rate of accumulation was higher in the liver. Cold stress resulted in a significantly higher induction of MT-1 than of MT-2, while heat shock had no effect on the expression of either gene. (C) 2001 Elsevier Science Inc. All rights reserved.