Molecular basis of female-specific odorant responses in Bombyx mori
Molecular basis of female-specific odorant responses in Bombyx mori
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DOI：
10.1016/j.ibmb.2008.11.002
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发表时间：
2009-03-01
影响因子：
3.8
通讯作者：
Newcomb, Richard D.
中科院分区：
文献类型：
--
作者：
Anderson, Alisha R.;Wanner, Kevin W.;Newcomb, Richard D.
许多蛾种的雄性和雌性在性行为上表现出重要的差异。该领域的研究主要集中在性信息素接受的男性特异性行为、电生理学和分子生物学方面。一级的行为;虽然像雄性特有的行为一样，它们可以为鳞翅目害虫的干预和管理提供机会，但研究得较少。此前，我们从家蚕（Bombyx mori）的基因组中鉴定出编码假定气味受体（ORs）的基因，其中一些基因在成年雌性触角中的稳态转录水平高于雄性。我们已经鉴定了其中一些OR的全长cDNA序列，并描述了一个新的OR，这是女性偏向进化枝的一部分。通过在Sf9细胞中的表达和钙成像分析，我们测试了一系列化合物激活最具女性偏见的or， BmOR19, BmOR30， BmOR45和BmOR47的能力。BmOR19对芳樟醇有反应，而BmOR45和BmOR47对苯甲酸> -苯乙醇>苯甲醛有反应。未发现BmOR30的激活配体。RNA原位杂交实验表明，BmOR19在雌性嗅觉感觉神经元中表达，而雌性嗅觉感觉神经元与第二个表达BmOR45和/或BmOR47的ORN位于同一感觉器官中。这些受体的活性和表达可能解释了雌性家蚕长感觉毛状体的电生理现象，之前发现每个感觉神经元含有一个萜烯（BmOR19）和一个苯甲酸（BmOR45, BmOR47）。植物挥发物如芳樟醇、苯甲酸、2-苯乙醇和苯甲醛是某些蛾子雌性产卵的线索。这些化合物也在从许多夜蛾物种的毛笔中提取的雄性产生的信息素混合物中被发现。毛笔状结构以前没有报道过，但我们在成年雄性毛笔状结构中发现了雌性毛笔状结构。据推测，BmOR19、BmOR45和BmOR47在家蚕的某些雌性特异性气味反应中起作用，如产卵和/或检测到一种尚未确定的雄性产生的性信息素。(C) 2008 Elsevier Ltd版权所有。
Males and females of many moth species exhibit important differences in sexual behaviours. Much research in this field has focused on the male-specific behaviour, electrophysiology and molecular biology of sex pheromone reception. Female-specific behaviours; have been less well studied although, like male-specific behaviours, they could provide opportunities for intervention and management of lepidopteram pests. Previously, we identified genes encoding putative odorant receptors (ORs) from the genome of the silkworm, Bombyx mori, some of which have higher levels of steady-state transcript in the antennae of adult females compared with males. We have identified the full-length cDNA sequences of some of these ORs and described a novel OR that is part of a female-biased clade. Using expression in Sf9 cells and a calcium-imaging assay, we tested a range of compounds for their ability to activate the most highly female-biased ORs, BmOR19, BmOR30, BmOR45 and BmOR47. BmOR19 responds to linalool, while BmOR45 and BmOR47 respond to benzoic acid > 2-phenylethanol > benzaldehyde. No activating ligands were found for BmOR30. RNA in situ hybridisation experiments reveal that BmOR19 is expressed in female olfactory sensory neurons that are co-located in the same sensilla as a second ORN expressing BmOR45 and/or BmOR47 Taken together the activity and expression of these receptors is likely explanatory of the observed electrophysiology of long sensilla trichoidea of female B. mori, previously shown to each contain one terpene (BmOR19) and one benzoic acid (BmOR45, BmOR47) sensory neuron. Plant volatiles such as linalool, benzoic acid, 2-phenylethanol and benzaldehyde are oviposition cues for females of some moths. These compounds have also been found in male-produced pheromone blends extracted from the hair pencils of many noctuid species. Hair pencil structures have not previously been reported for B. mori, but we have found hair pencil-like structures in adult male B. mori that are absent in female moths. It is proposed that BmOR19, BmOR45 and BmOR47 account for some of the female-specific odorant responses in B. mori, such as oviposition and/or detection of an as yet unidentified male-produced sex pheromone. (C) 2008 Elsevier Ltd. All rights reserved.