Protective effect of phenolic compounds isolated from the hooks and stems of uncaria sinensis on glutamate-induced neuronal death
Protective effect of phenolic compounds isolated from the hooks and stems of uncaria sinensis on glutamate-induced neuronal death
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DOI：
10.1142/s0192415x01000198
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发表时间：
2001-01-01
影响因子：
5.7
通讯作者：
Terasawa, K
中科院分区：
文献类型：
--
作者：
Shimada, Y;Goto, H;Terasawa, K
我们从钩藤（HSUS）的钩和茎中分离出酚类化合物表儿茶素、儿茶素、原花青素B-1、原花青素B-2、金丝桃苷和咖啡酸，并研究了它们对培养的大鼠小脑颗粒细胞中谷氨酸诱导的神经元死亡的保护作用。应用表儿茶素 (100-300 muM) 显着提高了通过 MTT 测定评估的细胞活力。儿茶素 (300 muM)、原花青素 B-1 (30-300 muM) 和原花青素 B-2 (100-300 muM) 与仅接触谷氨酸相比。表儿茶素(300μM)、儿茶素(300μM)、原花青素B-1(100-300μM)和原花青素B-2(100-300μM)也显着抑制由谷氨酸诱导的Ca-45(2+)流入细胞。这些结果表明，表儿茶素、儿茶素、原花青素 B-1 和原花青素 B-2 是 HSUS 的活性成分，可通过抑制 Ca2+ 内流来防止培养的小脑颗粒细胞中谷氨酸诱导的神经元死亡。
We isolated the phenolic compounds epicatechin, catechin, procyanidin B-1, procyanidin B-2, hyperin and caffeic acid from the hooks and stems of Uncaria sinensis (HSUS), and studied their protective effects against glutamate-induced neuronal death in cultured rat cerebellar granule cells. Cell viability evaluated by MTT assay was significantly increased by application of epicatechin (100-300 muM). catechin (300 muM), procyanidin B-1 (30-300 muM) and procyanidin B-2 (100-300 muM) compared with exposure to glutamate only. Ca-45(2+) influx into cells induced by glutamate was also significantly inhibited by administration of epicatechin (300 muM), catechin (300 uM), procyanidin B-1 (100-300 muM) and procyanidin B-2 (100-300 muM). These results suggest that epicatechin, catechin, procyanidin B-1 and procyanidin B-2 are the active components of HSUS that protect against glutamate-induced neuronal death in cultured cerebellar granule cells by inhibition of Ca2+ influx.