PREPARATION AND PURIFICATION OF LYMPHOCYTES FROM THE EPITHELIUM AND LAMINA PROPRIA OF MURINE SMALL-INTESTINE
PREPARATION AND PURIFICATION OF LYMPHOCYTES FROM THE EPITHELIUM AND LAMINA PROPRIA OF MURINE SMALL-INTESTINE
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DOI：
10.1136/gut.22.6.481
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发表时间：
1981-01-01
期刊：
影响因子：
24.5
通讯作者：
PARROTT, DMV
中科院分区：
文献类型：
--
作者：
DAVIES, MDJ;PARROTT, DMV
现有的从小肠生产淋巴细胞的方法在应用于小鼠时被证明是不令人满意的。报道了一种从小鼠小肠上皮层和固有层制备高纯度淋巴细胞悬液的新方法。详细介绍了该产品的生产和提纯方法。从每个小肠的上皮或固有层中至少可以获得1000万个淋巴细胞；细胞悬液几乎不受非淋巴样细胞的污染。对这两种细胞类型的初步分析表明，它们要么属于两个独立的群体，要么属于处于非常不同分化阶段的一个群体。使用来自小肠上皮和固有层的纯化淋巴细胞可以检查对肠上皮细胞的细胞毒性的产生[以及一般肠道的免疫学检查]；这在炎症性肠病的发展中可能是重要的。
Existing methods for the production of lymphocytes from the small intestine have proved unsatisfactory when applied to the mouse. A new method for the production of highly pure suspensions of lymphoid cells from the epithelial layer and lamina propria of mouse small intestine is reported. The production and purification methods are described in detail. At least 10 million lymphocytes are obtainable from each small intestine from either the epithelium or lamina propria; the cell suspensions are little contaminated by nonlymphoid cells. Preliminary analysis of the 2 cell types indicates that they belong either to 2 separate populations or to 1 population in very different stages of differentiation. The use of purified lymphoid cells from the epithelium and lamina propria of the small intestine may enable examination of the generation of cytotoxicity towards gut epithelial cells [and examination of the immunology of the gut in general]; this may be important in the development of inflammatory bowel diseases.