Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins
Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins
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DOI：
10.1016/s1046-2023(03)00006-9
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发表时间：
2003-05-01
期刊：
影响因子：
4.8
通讯作者：
Sakai, R
中科院分区：
文献类型：
--
作者：
Knöpfel, T;Tomita, K;Sakai, R
多年来，使用电压敏感染料对电活动进行光学成像一直被认为是研究脑组织信息处理的多神经元表示的有效方法。本文描述了新型基因靶向电压敏感荧光蛋白的出现。这种新型膜电压传感器克服了以前与传统电压敏感染料相关的膜非选择性染色相关的限制。在这里，我们讨论这种新技术的方法、应用和潜在优势。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique. (C) 2003 Elsevier Science (USA). All rights reserved.