β-cyclodextrin functionalized poly (5-amidoisophthalicacid) grafted Fe3O4 magnetic nanoparticles: A novel biocompatible nanocomposite for targeted docetaxel delivery
β-cyclodextrin functionalized poly (5-amidoisophthalicacid) grafted Fe3O4 magnetic nanoparticles: A novel biocompatible nanocomposite for targeted docetaxel delivery
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DOI：
10.1016/j.jmmm.2016.05.080
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发表时间：
2016-11-01
影响因子：
2.7
通讯作者：
Shafiee, Abbas
中科院分区：
文献类型：
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作者：
Tarasi, Roghayeh;Khoobi, Mehdi;Shafiee, Abbas
采用硫醇-内酰胺引发自由基聚合（TLIRP）法成功地制备了聚N-5-丙烯酰胺基丙酸接枝到Fe 3 O 4磁性纳米粒子（MNP @PAIP）上。然后通过碳二亚胺活化将β-环糊精（CD）缀合至所制备的MNP的羧基。随后，将肿瘤靶向叶酸（FA）连接到后者MNP表面CD的羟基上，以增加位点特异性细胞内递送。采用FTIR、VSM、TGA、XRD、FE-SEM和TEM等手段对所制备的MNP进行了表征。将疏水性抗癌药物多西紫杉醇（DTX）与CD通过主客体包合物进行负载，并研究了不同pH条件下系统的释放特性。对人胚肾正常细胞系（HEK 293）以及HeLa和MDA-MB-231癌细胞系的细胞活力进行了评价，结果未显示任何明显的细胞毒性作用。相比之下，负载DTX的MNP比游离DTX更多地减少HeLa和MDA-MB-231细胞的生长。细胞内摄取能力的DTX负载的MNP也进行了研究，使用荧光显微镜，并显示约90%的细胞摄取后，4小时的治疗。(C)2016爱思唯尔B. V.保留所有权利。
Thiol-lactam initiated radical polymerization (TLIRP) was successfully employed to prepare poly-N-5-acrylamidoisophthalicacid grafted onto Fe3O4 magnetic nanoparticles (MNPs@PAIP). beta-Cyclodextrin (CD) was then conjugated to the carboxylic groups of the prepared MNPs via carbodiimide activation. Subsequently, tumor-targeting folic acid (FA) was attached to the hydroxyl groups of CD on the surface of the latter MNPs to increase the site-specific intracellular delivery. The prepared MNPs were fully characterized by FTIR, VSM, TGA, XRD, FE-SEM and TEM. Docetaxel (DTX) as hydrophobic anticancer drug was loaded via host-guest inclusion complexation with CD and the release profile of the system was studied at different pH. The effect of MNPs on the cell viability was evaluated for the human embryonic kidney normal cell line (HEK293) as well as HeLa and MDA-MB-231 cancerous cell lines and the results did not show any apparent cytotoxic effect. In comparison, DTX loaded MNPs reduced the growth of HeLa and MDA-MB-231 cells more than free DTX. Intracellular uptake ability of DTX loaded MNPs was also studied using fluorescent microscopy and showed cellular uptake about 90% after 4 h treatment. (C) 2016 Elsevier B.V. All rights reserved.