Dendritic cells are dysfunctional in patients with operable breast cancer
Dendritic cells are dysfunctional in patients with operable breast cancer
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DOI：
10.1007/s00262-003-0485-5
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发表时间：
2004-06-01
影响因子：
5.8
通讯作者：
Eremin, O
中科院分区：
文献类型：
--
作者：
Satthaporn, S;Robins, A;Eremin, O
背景：树突状细胞（Dendritic cells，DCs）在向T淋巴细胞提呈抗原和诱导细胞毒性T细胞方面发挥重要作用。DC已在乳腺癌患者中进行了研究，以确定导致有效的全身和局部抗癌host respons.Methods失败的因素：纯化的DC从外周血（PB）和淋巴结（LN）的妇女与可手术的乳腺癌，使用免疫磁珠选择。结果：从外周血和淋巴结中分离出70-75%的纯化DC，并检测了DC在同种异体混合白细胞反应（MLR）和自体T细胞增殖试验（PPD为刺激剂）中的刺激活性、DC表面标志的表达和体外细胞因子的产生。与来自正常供体的PBDC相比，来自可手术乳腺癌患者的PBDC和LNDC在MLR中的刺激能力降低（p
Background: Dendritic cells (DCs) play a crucial role in presenting antigens to T lymphocytes and inducing cytotoxic T cells. DCs have been studied in patients with breast cancer to define the factors leading to failure of an effective systemic and locoregional anticancer host response.Methods: Purified DCs were obtained from peripheral blood (PB) and lymph nodes (LNs) of women with operable breast cancer, using immunomagnetic bead selection. The stimulatory capacity of DCs in the allogeneic mixed leukocyte reaction (MLR) and autologous T cell proliferation test (purified protein derivative (PPD) as stimulator), the expression of surface markers on DCs and the production of cytokines in vitro by DCs from patients with operable breast cancer and from healthy donors (controls) were studied.Results: 70-75% purified DCs were isolated from PB and LNs. PBDCs and LNDCs from patients with operable breast cancer demonstrated a reduced capacity to stimulate in an MLR, compared with PBDCs from normal donors (p