An association between plasma asymmetric dimethylarginine and membrane fluidity of erythrocytes in hypertensive and normotensive men: an electron paramagnetic resonance investigation.
An association between plasma asymmetric dimethylarginine and membrane fluidity of erythrocytes in hypertensive and normotensive men: an electron paramagnetic resonance investigation.
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高血压和血压正常男性血浆不对称二甲基精氨酸与红细胞膜流动性之间的关联：电子顺磁共振研究。
DOI：
10.1016/j.amjhyper.2005.04.007
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发表时间：
2005
影响因子：
3.2
通讯作者：
I. Nishio
中科院分区：
文献类型：
--
作者：
K. Tsuda;I. Nishio
研究背景有证据表明，一氧化氮（NO）合酶的内源性抑制剂不对称二甲基精氨酸（ADMA）水平的升高与血管功能障碍的风险增加有关。本研究旨在探讨高血压和正常血压男性血浆ADMA水平与膜流动性之间的可能关系。采用电子顺磁共振（EPR）和自旋标记方法测量了高血压男性（n= 38）和正常血压男性（n= 35）红细胞的膜微粘度（膜微粘度的倒数）。结果自旋标记剂的有序参数（S）高血压组红细胞膜5-硝基氧硬脂酸酯的EPR谱显著高于正常血压组（均数± SEM：高血压组，0.725 ± 0.001，n = 38;正常血压组，0.713 ± 0.001，n = 35，P<0.001），表明高血压组红细胞膜流动性低于正常血压组。高血压患者血浆NO代谢产物（亚硝酸盐和硝酸盐）浓度低于血压正常者。与此相反，血浆ADMA水平高血压男性高于血压正常的男性。在高血压和正常血压男性的总体分析中，红细胞的序参量（S）与血浆NO代谢产物呈负相关（r=-0.36，n = 73，P< .01），与血浆ADMA水平呈正相关（r = 0.44，n = 73，P<0.001），表明红细胞膜流动性降低与血浆NO水平降低和ADMA水平升高有关。当对高血压和正常血压男性分别进行相关分析时，两组血浆ADMA水平与红细胞有序参数（S）呈正相关。结论本研究的结果表明，高血压男性血浆ADMA水平升高，并与红细胞膜流动性降低有关，提示ADMA与高血压病红细胞流变性及微循环密切相关。Am J Hypertens 2005;18：1243-1248 © 2005 American Journal of Hypertension，Ltd.
BackgroundEvidence indicates that increased levels of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthase, are associated with increased risk of vascular dysfunction. The present study was performed to assess the possible relationship between plasma ADMA levels and membrane fluidity in hypertensive and normotensive men.MethodsMembrane fluidity (a reciprocal value of membrane microviscosity) of erythrocytes in hypertensive (n= 38) and normotensive (n= 35) men were measured using an electron paramagnetic resonance (EPR) and spin-labeling method.ResultsThe order parameter (S) for the spin-label agent (5-nitroxide stearate) in the EPR spectra of erythrocyte membranes was significantly higher in hypertensive men than in normotensive men (mean ± SEM: hypertensive subjects, 0.725 ± 0.001,n= 38; normotensive subjects, 0.713 ± 0.001,n= 35,P< .001), indicating that membrane fluidity of erythrocytes was decreased in hypertensive men compared with normotensive men. Plasma NO metabolite (nitrite and nitrate) concentration was lower in hypertensive than in normotensive men. In contrast, plasma ADMA levels were higher in hypertensive than in normotensive men. In the overall analysis of hypertensive and normotensive men, the order parameter (S) of erythrocytes was inversely correlated with plasma NO metabolites (r= −0.36,n= 73,P< .01) and positively correlated with plasma ADMA levels (r = 0.44,n= 73,P< .001), indicating that the reduced membrane fluidity of erythrocytes was associated with decreased NO and increased ADMA levels in plasma. When correlation analysis was performed separately for hypertensive and normotensive men, there was a positive correlation between plasma ADMA levels and the order parameter (S) of erythrocytes in both groups.ConclusionsThe results of the present study showed that the plasma ADMA levels were elevated in hypertensive men and associated with lower membrane fluidity of erythrocytes, suggesting that ADMA might have a close correlation with the rheologic behavior of erythrocytes and the microcirculation in hypertension. Am J Hypertens 2005;18:1243–1248 © 2005 American Journal of Hypertension, Ltd.
DOI：
10.1016/s0735-1097(00)01013-5
发表时间：
2000-12-01
影响因子：
24
作者：
Böger, RH;Bode-Böger, SM;Cooke, JP
通讯作者：
Cooke, JP
影响因子：
--
作者：
Lentz, SR;Rodionov, RN;Dayal, S
通讯作者：
Dayal, S