Murine norovirus: propagation, quantification, and genetic manipulation.
Murine norovirus: propagation, quantification, and genetic manipulation.
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DOI：
10.1002/9780471729259.mc15k02s33
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发表时间：
2014-05-01
影响因子：
--
通讯作者：
Wobus, Christiane E
中科院分区：
文献类型：
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作者：
Hwang, Seungmin;Alhatlani, Bader;Arias, Armando;Caddy, Sarah L;Christodoulou, Constantina;Cunha, Juliana Bragazza;Emmott, Ed;Gonzalez-Hernandez, Marta;Kolawole, Abimbola;Lu, Jia;Rippinger, Christine;Sorgeloos, Frederic;Thorne, Lucy;Vashist, Surender;Goodfellow, Ian;Wobus, Christiane E
鼠诺如病毒（MNV）是冠状病毒科的一种正链RNA病毒。它是生物医学研究菌落中最常见的病原体。MNV还与人类诺如病毒有关，诺如病毒是世界上大多数非细菌性胃肠炎的病因。与人类诺如病毒一样，MNV是一种肠道病毒，可在肠道内复制并通过粪-口途径传播。MNV在小鼠巨噬细胞和树突状细胞中复制，在培养细胞和小鼠宿主中复制。该病毒常用于研究诺如病毒生物学的机制，因为人类诺如病毒在细胞培养中难以生长。MNV结合了细胞培养和反向遗传系统的可用性以及研究原生宿主感染的能力。在这里，我们描述了一组通常用于研究MNV生物学的技术。
Murine norovirus (MNV) is a positive-sense, plus-stranded RNA virus in the Caliciviridae family. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of non-bacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal-oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because the human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology.