Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti
Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti
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DOI：
10.1371/journal.pone.0053816
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发表时间：
2013-01-14
期刊：
影响因子：
3.7
通讯作者：
Capurro, Margareth Lara
中科院分区：
文献类型：
--
作者：
Costa-da-Silva, Andre Luis;Navarrete, Flavia Rosa;Capurro, Margareth Lara
埃及伊蚊是登革热病毒的主要传播媒介，它需要血餐才能产卵。虽然活体动物仍然是实验室群体的主要血液来源，但也有许多人工喂食器可用。这些喂食器也是实验性Ae口腔感染的最佳方法。埃及伊蚊感染登革热病毒。然而，它们中的大多数都是昂贵或费力建造的。根据拉特式给料器的工作原理，利用传统的锥形管、甘油和Parafilm-M研制了一种简易的室内给料器。与活体血源小鼠比较，人工喂养组(充血51.3%)和小鼠喂养组(40.6%)之间的摄血效率没有显著差异(p=0.1189)。因此，本报告开发了一种易于组装且经济实惠的人工饲养器，命名为“甘油管”。这种简单高效的饲喂装置可以用常见的实验室材料建造，用于研究Ae。埃及伊蚊。
Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated "Glytube" was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti.