Field strains of Stemphylium vesicarium with a resistance to dicarboximide fungicides correlated with changes in a two-component histidine kinase
Field strains of Stemphylium vesicarium with a resistance to dicarboximide fungicides correlated with changes in a two-component histidine kinase
复制标题
DOI：
10.1007/s10658-010-9642-9
复制
发表时间：
2010-10-01
影响因子：
1.8
通讯作者：
Brunelli, Agostino
中科院分区：
文献类型：
--
作者：
Alberoni, Giulia;Collina, Marina;Brunelli, Agostino
根据该菌对敌草胺类杀菌剂的体外反应，可识别出4种表型：S(敏感)、S+(对扑菌丹和扑霉灵低抗性，但对长春草灵中等抗性)、R-1(中抗异扑菌酮和扑草灵，但高度抗扑霉胺)、R-2(对所有杀菌剂高度抗药性)。所有病例对二甲亚胺和芳香烃类杀菌剂均有交叉耐药性，而对苯基吡咯的交叉耐药性仅在R-2表型中检测到。此外，对氧化和渗透应激诱导剂的敏感性没有发现变化。从泡囊茎瘤菌的几个分离株中获得了一个与粗脉孢子菌OS1同源的渗透敏感组氨酸激酶基因的序列。该基因编码1,329个氨基酸，在C-末端含有一个保守的组氨酸-激酶结构域，在N-末端有6个串联重复序列，约90个氨基酸。在S+和R-1表型分离株中，第一个氨基酸重复序列分别为F267L和L290S，存在单一氨基酸替换。对于R-2分离株，T765R或Q777R的交换位于组氨酸-激酶区。
In Stemphylium vesicarium, four phenotypes were recognized according to their in vitro responses to dicarboximide fungicides: S (sensitive), S+ (low resistant to iprodione and procymidone but moderately resistant to vinclozolin), R-1 (moderately resistant to iprodione and vinclozolin but highly resistant to procymidone), R-2 (highly resistant to all dicarboximides). Cross-resistance was observed between dicarboximides and aromatic hydrocarbon fungicides in all cases while cross-resistance to phenylpyrroles was only detected in R-2 phenotype. Moreover, no changes were noted in sensitivity to oxidative and osmotic stress inducers. An osmosensing histidine kinase gene, homologous to OS1 from Neurospora crassa, was sequenced from several field isolates of Stemphylium vesicarium. This gene is predicted to encode a 1,329 amino acid protein, comprising a conserved histidine-kinase domain in the C-terminal region and six tandem repeats of about 90 amino acids at the N-terminal end. In S+ and R-1 phenotype isolates, a single amino acid substitution was observed in the first amino acid repeat; F267L and L290S respectively. For the R-2 isolates, the exchanges T765R or Q777R were located within the histidine-kinase domain.