Repeatability and Reproducibility of Retinal Blood Flow Measurement Using a Doppler Optical Coherence Tomography Flowmeter in Healthy Subjects
Repeatability and Reproducibility of Retinal Blood Flow Measurement Using a Doppler Optical Coherence Tomography Flowmeter in Healthy Subjects
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DOI：
10.1167/iovs.16-21389
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发表时间：
2017-06-01
影响因子：
4.4
通讯作者：
Yoshida, Akitoshi
中科院分区：
文献类型：
--
作者：
Tani, Tomofumi;Song, Young-Seok;Yoshida, Akitoshi
目的.目的：评价在市售多普勒光学相干断层扫描（DOCT）系统中使用新的自动校准和测量软件测量视网膜血流（RBF）的重复性和再现性。DOCT流量计评估了RBF测量的会话内可重复性以及会话间和观察者间可重复性。对于序列内可重复性，计算了20名健康志愿者20只正常眼睛的视网膜动脉和静脉的五次重复RBF测量的变异系数（CV）。对于间期重现性，比较了由一名观察员在2个不同日期获得的两组三次测量值。对于观察者间的重现性，比较了两组由两名观察者在同一天获得的三个测量值。组内相关系数（ICC）也被用来评估重复性和再现性。DOCT流量计和激光多普勒测速仪（LDV）的相关性也进行了评估。对于插入重复性，RBF的ICC在小动脉和小静脉中超过0.90（ICC：分别为0.994和0.970）。小动脉和小静脉中RBF的CV分别为6.0% +/- 3.4%和8.8% +/-5.1%。在小动脉（ICC：分别为0.980和0.993）和小静脉（ICC：分别为0.982和0.986）中，会话间和观察者间RBF值具有较高的重现性。DOCT血流计测量的RBF与小动脉LDV呈强相关（r = 0.76; P < 0.001）。DOCT流量计在小动脉和小静脉中具有良好的再现性，并且与小动脉中的LDV相比，精确地测量RBF。
PURPOSE. To evaluate the repeatability and reproducibility of retinal blood flow (RBF) measurements in humans by using new auto-alignment and measurement software in a commercially available Doppler optical coherence tomography (DOCT) system.METHODS. The DOCT flowmeter assessed the intrasession repeatability and the intersession and interobserver reproducibility of the RBF measurements. For intrasession repeatability, the coefficients of variation (CVs) of five repeated RBF measurements were calculated at the retinal arteries and veins in 20 normal eyes of 20 healthy volunteers. For intersession reproducibility, two sets of three measurements obtained by one observer on 2 different days were compared. For interobserver reproducibility, two sets of three measurements obtained by two observers on the same day were compared. Intraclass correlation coefficients (ICCs) also were used to evaluate the repeatability and reproducibility. The relevance of the DOCT flowmeter and laser Doppler velocimetry (LDV) also was assessed.RESULTS. Regarding intrasession repeatability, the ICC of the RBF exceeded 0.90 in arterioles and venules (ICC: 0.994 and 0.970, respectively). The CVs of the RBF in the arterioles and venules were 6.0% +/- 3.4% and 8.8% +/- 5.1%, respectively. The intersession and interobserver RBF values had high reproducibility in the arterioles (ICC: 0.980 and 0.993, respectively) and venules (ICC: 0.982 and 0.986, respectively). The RBF measured with the DOCT flowmeter was correlated strongly with LDV in the arterioles (r = 0.76; P < 0.001).CONCLUSIONS. The DOCT flowmeter had good reproducibility in the arterioles and venules and precisely measured the RBF as compared to the LDV in the arterioles.