Expression of ephrinB2 and its receptors on fibroproliferative membranes in ocular angiogenic diseases
Expression of ephrinB2 and its receptors on fibroproliferative membranes in ocular angiogenic diseases
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DOI：
10.1016/j.ajo.2004.04.006
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发表时间：
2004-08-01
影响因子：
4.2
通讯作者：
Oshima, K
中科院分区：
文献类型：
--
作者：
Umeda, N;Ozaki, H;Oshima, K
目的：研究ephrinB2和EphB受体在视网膜纤维增生性膜上的表达，以确定ephrinB2是否在眼部血管生成中起作用。设计：玻璃体切除术中收集的眼部血管生成疾病患者纤维增生性膜内ephrinB2和EphB受体表达的实验研究。方法：从20例增殖性糖尿病视网膜病变（PDR）患者和40例早产儿5期视网膜病变患者玻璃体切除术时获得纤维增生性膜。使用针对ephrinB2和EphB2、EphB3和EphB4受体的多克隆抗体对标本进行免疫组化研究。测定血管性血液病因子（VIII因子）和α -平滑肌肌动蛋白（α - sma）的免疫反应性，以确认靶血管内皮细胞的身份。结果：在PDR患者（65.0%）和早产儿视网膜病变患者（25.0%）的纤维增生性膜上观察到ephrinB2阳性染色。具体来说，ephrinB2被发现存在于内皮细胞上，通过因子VIII和α - sma染色证实了它的共定位。在PDR患者（EphB2, 90.0%; EphB3, 70.0%）和早产儿视网膜病变患者（EphB2, 35.0%; EphB3, 45.0%）的纤维增生性膜上观察到EphB2和EphB3的表达。然而，在PDR或早产儿视网膜病变患者的任何膜中均未观察到EphB4表达。PDR患者的ephrinB2表达率明显高于早产儿视网膜病变患者，这可能反映了两者纤维增生性膜血管密度的差异。结论：这些数据提示ephrinB2-EphB2/B3系统可能在眼部血管生成中起重要作用。(C) 2004年Elsevier Inc.版权所有。
PURPOSE: To determine whether ephrinB2 plays a role in ocular angiogenesis, we investigated the expression of ephrinB2 and EphB receptors on retinal fibroproliferative membranes.DESIGN: Experimental study of the expression of ephrinB2 and EphB receptors within fibroproliferative membranes in patients with ocular angiogenic diseases collected during vitrectomy.METHODS: Fibroproliferative membranes were obtained at the time of vitrectomy from 20 patients with proliferative diabetic retinopathy (PDR) and from 40 patients who had stage 5 retinopathy of prematurity. Specimens were investigated with immunohistochemistry using polyclonal antibodies directed against ephrinB2 and the EphB2, EphB3, and EphB4 receptors. Immunoreactivity for von Willebrand factor (factor VIII) and alpha-smooth muscle actin (alpha-SMA) was also determined to confirm the identity of the target vascular endothelial cells.RESULTS: Positive staining for ephrinB2 was observed on fibroproliferative membranes that were obtained from patients with PDR (65.0%) and retinopathy of prematurity (25.0%). Specifically, ephrinB2 was found to be present on endothelial cells, as confirmed by its colocalization with factor VIII and alpha-SMA staining. EphB2 and EphB3 expression was observed on fibroproliferative membranes that were harvested from patients with PDR (EphB2, 90.0%; EphB3, 70.0%) and retinopathy of prematurity (EphB2, 35.0%; EphB3, 45.0%). However, EphB4 expression was not observed in any of the membranes derived from patients with PDR or retinopathy of prematurity. The rate of ephrinB2 expression in patients with PDR was significantly higher than that seen in patients with retinopathy of prematurity, which probably reflected differences in the vascular density of their fibroproliferative membranes.CONCLUSION: These data suggest that the ephrinB2-EphB2/B3 system may play an important role in ocular angiogenesis. (C) 2004 by Elsevier Inc. All rights reserved.