Nuclear Pore Complex Component MOS7/Nup88 Is Required for Innate Immunity and Nuclear Accumulation of Defense Regulators in Arabidopsis
Nuclear Pore Complex Component MOS7/Nup88 Is Required for Innate Immunity and Nuclear Accumulation of Defense Regulators in Arabidopsis
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DOI：
10.1105/tpc.108.064519
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发表时间：
2009-08-01
期刊：
影响因子：
11.6
通讯作者：
Li, Xin
中科院分区：
文献类型：
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作者：
Cheng, Yu Ti;Germain, Hugo;Li, Xin
植物的免疫应答依赖于通过核孔穿过核膜的动态信号事件。某些抗性（R）蛋白和下游信号转导子的核积累对其功能至关重要，但尚不清楚这些过程是如何控制的。在这里，我们报告的识别，克隆和分析的拟南芥修改器的snc 1，7（mos 7 -1），一个部分丧失功能的突变，抑制免疫反应条件下的自激活R蛋白snc 1（抑制npr 1 -1，组成1）。mos 7 -1单突变体植物在基础和R蛋白介导的免疫和系统获得性抗性方面表现出缺陷，但在发育、耐盐性或植物激素反应方面不表现出明显的多效性缺陷。MOS 7与人和果蝇核孔蛋白Nup 88同源，位于核膜上。在动物中，Nup 88减弱激活的NF-κ B转录因子的核输出，导致NF-κ B的核积聚。我们的分析表明，核积累的snc 1和防御信号组件增强疾病易感性1和非表达PR基因1显着减少mos 7 -1植物，而核保留的其他测试蛋白质不受影响。这些数据表明，特异性地调节某些防御蛋白的核浓度可以调节防御输出。
Plant immune responses depend on dynamic signaling events across the nuclear envelope through nuclear pores. Nuclear accumulation of certain resistance (R) proteins and downstream signal transducers are critical for their functions, but it is not understood how these processes are controlled. Here, we report the identification, cloning, and analysis of Arabidopsis thaliana modifier of snc1,7 (mos7-1), a partial loss-of-function mutation that suppresses immune responses conditioned by the autoactivated R protein snc1 (for suppressor of npr1-1, constitutive 1). mos7-1 single mutant plants exhibit defects in basal and R protein-mediated immunity and in systemic acquired resistance but do not display obvious pleiotropic defects in development, salt tolerance, or plant hormone responses. MOS7 is homologous to human and Drosophila melanogaster nucleoporin Nup88 and resides at the nuclear envelope. In animals, Nup88 attenuates nuclear export of activated NF-kappa B transcription factors, resulting in nuclear accumulation of NF-kappa B. Our analysis shows that nuclear accumulation of snc1 and the defense signaling components Enhanced Disease Susceptibility 1 and Nonexpresser of PR genes 1 is significantly reduced in mos7-1 plants, while nuclear retention of other tested proteins is unaffected. The data suggest that specifically modulating the nuclear concentrations of certain defense proteins regulates defense outputs.