AUTORADIOGRAPHIC ANALYSIS OF DNA AND RNA SYNTHESIS IN THE RED BLOOD CELLS OF THE DEVELOPING CHICK EMBRYO.
AUTORADIOGRAPHIC ANALYSIS OF DNA AND RNA SYNTHESIS IN THE RED BLOOD CELLS OF THE DEVELOPING CHICK EMBRYO.
复制标题
发育中的鸡胚胎红细胞中 DNA 和 RNA 合成的放射自显影分析。
DOI：
10.1016/0014-4827(64)90012-6
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发表时间：
1964
影响因子：
3.7
通讯作者：
R. Fraser
中科院分区：
文献类型：
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作者：
R. Fraser
用体外条件下的放射自显影技术，证明5日龄鸡胚的某些红细胞在DNA合成中摄取~ 3 H-胸苷，基本上所有红细胞都摄取~ 3 H-尿苷作为RNA前体，在某些定型红细胞系的早期细胞中，有相当多的DNA合成。这似乎令人惊讶，因为这些细胞没有被发现进行有丝分裂。在14天胚胎的红细胞中发现了一个弱标记，在5天胚胎的血细胞中发现RNA标记首先发生在细胞核中，然后随着时间的推移扩散到细胞质中。在成熟的原始红细胞中未观察到~ 3 H-尿嘧啶核苷的摄取，而在定型系的早期多染红细胞中有明显的~ 3 H-尿嘧啶核苷的摄取。本文讨论了体外实验中观察到的有关鸡胚红细胞发育过程中总合成能力的意义。
By the technique of autoradiography underin vitroconditions it has been possible to demonstrate that some of the red blood cells of the 5-day chick embryo take up3H-thymidine in the synthesis of DNA and essentially all of them take up3H-uridine as a RNA precursor.There is a rather great synthesis of DNA in certain early cells of the definitive erythrocyte line. This seems surprising, because these cells have not been found to undergo mitosis. A weak label was found in red blood cells from embryos as old as 14 days of incubation.RNA labeling was found to occur first in the nucleus, then spread into the cytoplasm with time in the blood cells of the 5-day embryo. No uptake of3H-uridine was observed in mature primitive erythrocytes, but was apparent in the early polychromatic cells of the definitive line.The significance of the observations in thesein vitroexperiments in relation to the total synthetic capacity of the erythrocytes of the chick embryo during development is discussed.