[Recent progresses in CRISPR genome editing in plants].
[Recent progresses in CRISPR genome editing in plants].
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DOI：
10.13345/j.cjb.170171
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发表时间：
2017-10-25
期刊：
影响因子：
--
通讯作者：
Xie, Kabin
中科院分区：
文献类型：
--
作者：
Li, Hong;Xie, Kabin
在过去的4年里，CRISPR/Cas9介导的基因组编辑成为生命科学中的革命性工具。这个工具使我们能够以前所未有的吞吐量、可扩展性、速度和低成本编辑植物基因。除了靶向敲入和敲除应用外，CRISPR/Cas9还为靶向基因激活和抑制提供了一个有效的平台。同时，CRISPR/Cas9基因组编辑的准确性、容量和效率也得到了提高，以进行复杂的基因操作。此外，基因组编辑工具箱还通过CRISPR/Cpf1介导的基因组编辑和单碱基编辑等新技术进行了扩展。由于这些最新进展，CRISPR技术接近植物科学的梦想工具，并将通过精确的基因组编辑加速作物遗传改良。
In the past 4 years, CRISPR/Cas9-mediated genome editing becomes the revolutionary tool in life sciences. This tool enables us to edit plant genes with unprecedented throughput, scalability, speed and low cost. In addition to targeted knock-in and knock-out applications, CRISPR/Cas9 also provides an efficient platform for targeted gene activation and suppression. At the same time, accuracy, capacity and efficiency of CRISPR/Cas9 genome editing have been improved for sophisticated genetic manipulation. Furthermore, the genome editing toolbox is expanded by new technologies like CRISPR/Cpf1-mediated genome editing and single base editing. Owing to these recent progresses, CRISPR technology is close to the dream tool for plant sciences and will accelerate the crop genetic improvement through precise genome editing.