CLONED MURINE LYMPHOCYTES-T SYNTHESIZE A MOLECULE WITH THE BIOLOGICAL CHARACTERISTICS OF NITRIC-OXIDE
CLONED MURINE LYMPHOCYTES-T SYNTHESIZE A MOLECULE WITH THE BIOLOGICAL CHARACTERISTICS OF NITRIC-OXIDE
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DOI：
10.1016/s0006-291x(05)80086-5
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发表时间：
1990-12-14
影响因子：
3.1
通讯作者：
BARBUL, A
中科院分区：
文献类型：
--
作者：
KIRK, SJ;REGAN, MC;BARBUL, A
最近，活化的中性粒细胞和巨噬细胞被证明可以完全由l -精氨酸合成一氧化氮（NO）。我们在小鼠T细胞克隆中寻找这一途径的存在。利用对NO敏感的血小板聚集生物测定，我们证明了il -2刺激的CTLL和HT2细胞抑制血小板聚集，而未刺激的淋巴细胞则没有。这一作用可被特定的NO合成酶竞争对手ng -单甲基精氨酸抑制，只有L型精氨酸或其类似物L-同型精氨酸能够为NO合成提供底物。
Recently, activated neutrophils and macrophages have been shown to synthesize nitric oxide (NO) exclusively from L-arginine. We searched for the presence of this pathway in murine T cell clones. Using a platelet aggregation bioassay sensitive to NO, we demonstrate that IL-2-stimulated CTLL and HT2 cells inhibit platelet aggregation, whereas unstimulated lymphocytes do not. This action can be inhibited by the specific NO synthase competitor NG-mono-methyl arginine, and only the L form of arginine or its analogue L-homoarginine are capable of providing substrate for NO synthesis.