Leydig cell transplantation restores androgen production in surgically castrated prepubertal rats.
Leydig cell transplantation restores androgen production in surgically castrated prepubertal rats.
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DOI：
10.1038/aja.2009.22
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发表时间：
2009-05
影响因子：
2.9
通讯作者：
Jie Sun;Y. Xi;Zhong-de Zhang;P. Shen;Huai-Yuan Li;M. Yin;Wei-yi Li;C. Shi
中科院分区：
文献类型：
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作者：
Jie Sun;Y. Xi;Zhong-de Zhang;P. Shen;Huai-Yuan Li;M. Yin;Wei-yi Li;C. Shi
据估计，全世界每 200 名儿童中就有 1 人患有青春期前睾丸功能障碍以及随后发生的性腺功能减退症。由于睾酮水平在发育和青春期期间是动态的，传统的激素治疗方案往往不足，从而导致相关的生理状况得不到解决。因此，我们通过使用手术诱导的性腺功能减退症大鼠模型系统，研究了成熟 Leydig 细胞移植治疗青春期前原发性性腺功能减退症的潜在治疗效果。在实验中，通过手术从成熟的 Sprague-Dawley 大鼠中分离出 Leydig 细胞，并将其移植到青春期前的受体中。将血清睾酮水平和染色睾丸间质的显微镜分析与假处理的对照以及性发育过程中的阉割和完整大鼠进行比较。植入后 4 周，在 Leydig 细胞受体中可检测到血清睾酮，但在手术对照组中未检测到，并且随着时间的推移逐渐增加，直至在植入后 12 周达到与性成熟男性相当的水平。组织学分析揭示了间质细胞的高存活率以及类固醇分泌活性。因此，我们得出结论，青春期前性腺功能减退症受者的成熟Leydig细胞移植对大鼠具有治疗潜力，值得进一步研究用于临床应用。
Prepubertal testicular dysfunction and the subsequent development of hypogonadism affects an estimated one in 200 children worldwide. As the testosterone levels are dynamic during development and puberty, traditional hormone treatment regimens are often inadequate, thereby leaving associated physiological conditions unresolved. Therefore, we have investigated the potential therapeutic effect of mature Leydig cell transplantation for the treatment of prepubertal primary hypogonadism through the use of a surgically induced hypogonadistic rat model system. In the experiment, Leydig cells were surgically isolated from mature Sprague–Dawley rats and transplanted into prepubertal recipients. Serum testosterone levels and microscopic analysis of the stained testicular interstitium were compared with sham-treated controls, as well as with castrated and intact rats during sexual development. At 4 weeks post-implantation, serum testosterone was detectable in Leydig cell recipients, but not in surgical controls, and progressively increased as a function of time until reaching levels comparable with sexually mature males at 12 weeks post-implantation. Histological analysis revealed a high rate of Leydig cell survival as well as steroidogenic secretory activity. Therefore, we conclude that mature Leydig cell transplantation in prepubertal hypogonadism recipients has therapeutic potential in rats and merits further investigation for clinical application.