Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates
Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates
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DOI：
10.1007/s00253-007-1144-0
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发表时间：
2007-09
影响因子：
5
通讯作者：
Hiroshi Yokoyama;N. Moriya;H. Ohmori;M. Waki;A. Ogino;Yasuo Tanaka
中科院分区：
文献类型：
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作者：
Hiroshi Yokoyama;N. Moriya;H. Ohmori;M. Waki;A. Ogino;Yasuo Tanaka
采用变性梯度凝胶电泳（DGGE）和克隆文库分析两种不依赖培养的方法，对极端高温条件下厌氧产氢菌群的群落结构进行了分析。极端嗜热菌群（ETM）通过在75°C下使用木糖、葡萄糖、乳糖、纤维二糖或可溶性淀粉的底物通过重复分批培养从牛粪中富集，并且分别以0.56、2.65、2.17、2.68和1.73 mol/mol单糖降解的产率产生氢气。DGGE和克隆文库分析的结果是一致的，并表明，ETM的社区结构丰富的四个己糖基底物（葡萄糖，乳糖，纤维二糖，可溶性淀粉）由一个单一的物种，密切相关的产氢极端嗜热菌，Caldoanaerobacterium subarachieus，在亚种水平的多样性。富含木糖的ETM比富含其他底物的ETM更多样化，并且含有与C. subacrylus和一种未分类的细菌，与一种降解木聚糖和产氢的极端嗜热菌Caloramator fervidus有远亲关系。
The present study analyzed the community structures of anaerobic microflora producing hydrogen under extreme thermophilic conditions by two culture-independent methods: denaturing gradient gel electrophoresis (DGGE) and clone library analyses. Extreme thermophilic microflora (ETM) was enriched from cow manure by repeated batch cultures at 75°C, using a substrate of xylose, glucose, lactose, cellobiose, or soluble starch, and produced hydrogen at yields of 0.56, 2.65, 2.17, 2.68, and 1.73 mol/mol-monosaccharide degraded, respectively. The results from the DGGE and clone library analyses were consistent and demonstrated that the community structures of ETM enriched with the four hexose-based substrates (glucose, lactose, cellobiose, and soluble starch) consisted of a single species, closely related to a hydrogen-producing extreme thermophile,Caldoanaerobacter subterraneus, with diversity at subspecies levels. The ETM enriched with xylose was more diverse than those enriched with the other substrates, and contained the bacterium related toC. subterraneusand an unclassified bacterium, distantly related to a xylan-degrading and hydrogen-producing extreme thermophile,Caloramator fervidus.