SPECTINOMYCIN RESISTANCE AT SITE-1192 IN 16S-RIBOSOMAL RNA OF ESCHERICHIA-COLI - AN ANALYSIS OF 3 MUTANTS
SPECTINOMYCIN RESISTANCE AT SITE-1192 IN 16S-RIBOSOMAL RNA OF ESCHERICHIA-COLI - AN ANALYSIS OF 3 MUTANTS
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DOI：
10.1016/0300-9084(87)90216-1
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发表时间：
1987-08-01
期刊：
影响因子：
3.9
通讯作者：
DAHLBERG, AE
中科院分区：
文献类型：
--
作者：
MAKOSKY, PC;DAHLBERG, AE
利用定点突变技术在大肠杆菌质粒编码的rrnB操纵子上的16 S rRNA残基C-1192处构建了三个不同的单碱基替换。所有3种突变体在转化细胞中赋予不同水平的对壮观霉素的抗性，但在不存在抗生素的情况下均不影响生长速率。G-1192突变体赋予显著的抗性，允许在40 mg/ml的壮观霉素中生长。
Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of Escherichia coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.