NDM-1-and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012
NDM-1-and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012
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DOI：
10.1016/j.jgar.2013.06.003
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发表时间：
2013-12-01
影响因子：
4.6
通讯作者：
Mammina, Caterina
中科院分区：
文献类型：
--
作者：
Abdelaziz, Mohammed O.;Bonura, Celestino;Mammina, Caterina
在这里，我们描述了从埃及开罗一家癌症医院的同一重症监护病房的两名住院患者中分离出的两株肺炎克雷伯菌的碳青霉烯类耐药决定因素。采用PCR和测序技术检测和鉴定β -内酰胺酶基因。采用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）分析了分离株间的克隆关系。第一株肺炎克雷伯菌分离株携带bla（NDm-1）基因，第二株分离株携带bla（oxA-163）基因。两株菌株共表达广谱β -内酰胺酶CTX-M-15。两个分离株分别属于ST11和ST16两种不同的序列类型。两名患者均无旅行史。埃及首次发现产生ndm -1的肺炎克雷伯菌，进一步证明产生ndm -1的革兰氏阴性微生物在北非传播。在肺炎克雷伯菌分离物中额外检测到bla(oxA-163)，证实其在该地理区域的关键卫生保健环境中存在地方性疾病。(C) 2013年国际感染与肿瘤化疗学会。Elsevier Ltd.出版。版权所有。
Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise beta-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the bla(NDm-1) gene and the second isolate carried the bla(oxA-163) gene. Both isolates co-expressed the extended-spectrum beta-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of travel was established for the two patients. The first identification of NDM-1-producing K. pneumoniae in Egypt adds further evidence to the spread of NDM-1-producing Gram-negative micro-organisms in North Africa. The additional detection of bla(oxA-163) in a K. pneumoniae isolate confirms its endemic presence in a critical healthcare setting of this geographic area. (C) 2013 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.