RAPID KINETIC MEASUREMENTS OF 2ND MESSENGER FORMATION IN OLFACTORY CILIA FROM CHANNEL CATFISH
RAPID KINETIC MEASUREMENTS OF 2ND MESSENGER FORMATION IN OLFACTORY CILIA FROM CHANNEL CATFISH
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DOI：
10.1152/ajpcell.1993.264.4.c906
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发表时间：
1993-04-01
影响因子：
--
通讯作者：
BREER, H
中科院分区：
文献类型：
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作者：
RESTREPO, D;BOEKHOFF, I;BREER, H
使用有味氨基酸刺激斑点叉尾鮰 (Ictalurus punctatus) 的嗅觉纤毛对腺苷 3',5'-环单磷酸、鸟苷 3',5'-环单磷酸和肌醇 1,4,5-三磷酸 (IP30) 形成的影响，使用淬火流技术在亚秒级时间范围内进行了研究。L-丙氨酸 (L-Ala) 和 L-半胱氨酸(L-CYS) (100 muM) 会引起 IP3 水平短暂升高，并在 25 ms 时达到峰值。相比之下，即使在高浓度下，有味氨基酸（1 MM L-Ala、L-CYS、L-正亮氨酸、L-谷氨酸、L-脯氨酸和 L-精氨酸）在此时间范围内也不会引起 cAMP 水平的变化，并且仅导致 cGMP 相对缓慢且较小的增加。 L-Cys 和 L-Ala 在 10 nM 至 1 mM 范围内的 IP3 反应的剂量-反应关系与之前的电生理学和配体结合实验一致，气味氨基酸刺激 IP 的形成是 GTP 依赖性的，并且被鸟苷 5'-O-(2-硫代二磷酸) 抑制，表明该反应是 G 蛋白介导的。鲶鱼中的转导。
The effect of stimulating olfactory cilia from the channel catfish (Ictalurus punctatus) with odorant amino acids on the formation of adenosine 3',5'-cyclic monophosphate, guanosine 3',5'-cyclic monophosphate, and inositol 1,4,5-trisphosphate (IP30 was studied in the subsecond time scale using a quenched flow technique. L-Alanine (L-Ala) and L-cysteine (L-CYS) (100 muM) elicited a transient elevation in IP3 levels that peaked at 25 ms. In contrast, even at high concentration a mixture of odorant amino acids (1 MM L-Ala, L-CYS, L-norleucine, L-glutamate, L-proline, and L-arginine) did not elicit a change in cAMP levels in this time scale and caused only relatively slow and minor increases in cGMP. The dose-response relationship for the IP3 response of L-Cys and L-Ala in the range from 10 nM to 1 mM is consistent with previous electrophysiological and ligand binding experiments. Odorant amino acid-stimulated IP, formation was GTP dependent and was inhibited by guanosine 5'-O-(2-thiodiphosphate), suggesting that the response was G protein mediated. These results are consistent with a mediatory role for IP3 in amino acid olfactory signal transduction in catfish.