Integrated proteomics and metabolomics analysis of tea leaves fermented by Aspergillus niger, Aspergillus tamarii and Aspergillus fumigatus
Integrated proteomics and metabolomics analysis of tea leaves fermented by Aspergillus niger, Aspergillus tamarii and Aspergillus fumigatus
复制标题
黑曲霉、塔玛丽曲霉和烟曲霉发酵茶叶的蛋白质组学和代谢组学综合分析。
DOI：
10.1016/j.foodchem.2020.127560
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发表时间：
2021-01-01
期刊：
影响因子：
8.8
通讯作者：
Zhao, Ming
中科院分区：
文献类型：
--
作者：
Ma, Yan;Ling, Tie-jun;Zhao, Ming
后发酵普洱茶（PFPT）是一种具有独特感官品质和多种保健功效的微生物发酵茶。曲霉是发酵过程中的优势真菌，也是PFPT特性的主要贡献者，因此其潜在功能值得详细研究。在这里，茶叶发酵尼日尔曲霉，曲霉Tamarii和烟曲霉，并得到的样品（指定为Asn，Ast和Asf，分别）进行了分析，通过蛋白质组学和代谢组学方法。曲霉发酵产生的黄酮类化合物，甘油磷脂，有机含氧化合物和脂肪酸的组成的变化进行了观察。碳水化合物活性酶，例如，鉴定了用于降解纤维素、淀粉、木质素、果胶、木聚糖和木葡聚糖的内切葡聚糖酶和纤维素酶。糖苷水解酶、糖基转移酶、单宁酶、漆酶、香草醇氧化酶和苯醌还原酶被鉴定并推测催化酚类化合物的水解、氧化、聚合和降解。总之，茶曲霉的功能被证明是产生酶来改变茶叶中代谢物的浓度和组成。
Post-fermented Pu-erh tea (PFPT) is a microbially-fermented tea with distinct sensory qualities and multiple health benefits. Aspergillus are the dominant fungi in the fermentation and the main contributors to the characteristics of PFPT, so their underlying functions warrant detailed study. Here, tea leaves were fermented by Aspergillus niger, Aspergillus tamarii and Aspergillus fumigatus, and resulting samples (designated as Asn, Ast and Asf, respectively) were analyzed by proteomic and metabolomic methods. Changes to the composition of flavonoids, glycerophospholipids, organo-oxygen compounds and fatty acids resulting from Aspergillus fermentation were observed. Carbohydrate-active enzymes, e.g., endoglucanases and cellulases, for degradation of cellulose, starch, lignin, pectin, xylan and xyloglucan were identified. Glycoside hydrolase, glycosyltransferases, tannase, laccases, vanillyl-alcohol oxidases and benzoquinone reductase were identified and hypothesized to catalyze hydrolysis, oxidation, polymerization and degradation of phenolic compounds. Together, functions of Aspergillius were demonstrated as production of enzymes to change concentrations and compositions of metabolites in tea leaves.