Physical and Functional Compartmentalization of Archaeal Chromosomes
Physical and Functional Compartmentalization of Archaeal Chromosomes
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DOI：
10.1016/j.cell.2019.08.036
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发表时间：
2019-09-19
期刊：
影响因子：
64.5
通讯作者：
Bell, Stephen D.
中科院分区：
文献类型：
--
作者：
Takemata, Naomichi;Samson, Rachel Y.;Bell, Stephen D.
染色体的三维组织可以对其复制和表达产生深远的影响。高等真核生物的染色体具有离散的区室，其特征在于不同的转录活性。相比之下，大多数细菌染色体具有更简单的局部结构域，其边界受基因表达的影响。大量研究表明，细菌和真核生物染色体的高阶结构依赖于染色体结构维持（SMC）超家族蛋白复合物的作用，特别是近通用凝聚素复合物。然而，有趣的是，许多古细菌，包括硫化叶菌属的成员，并不编码典型的凝聚素。我们描述了硫化叶菌属物种的染色体构象捕获实验。这些揭示了存在不同的域沿着硫化叶菌染色体进行离散和特定的高阶相互作用，从而定义两个隔间类型。我们观察到一个未被描述的SMC超家族蛋白，我们称之为“聚结蛋白”，在区室身份、基因表达和结合之间存在因果联系。"
The three-dimensional organization of chromosomes can have a profound impact on their replication and expression. The chromosomes of higher eukaryotes possess discrete compartments that are characterized by differing transcriptional activities. Contrastingly, most bacterial chromosomes have simpler organization with local domains, the boundaries of which are influenced by gene expression. Numerous studies have revealed that the higher-order architectures of bacterial and eukaryotic chromosomes are dependent on the actions of structural maintenance of chromosomes (SMC) superfamily protein complexes, in particular, the near-universal condensin complex. Intriguingly, however, many archaea, including members of the genus Sulfolobus do not encode canonical condensin. We describe chromosome conformation capture experiments on Sulfolobus species. These reveal the presence of distinct domains along Sulfolobus chromosomes that undergo discrete and specific higher-order interactions, thus defining two compartment types. We observe causal linkages between compartment identity, gene expression, and binding of a uncharacterized SMC superfamily protein that we term "coalescin."