The aerobic energy metabolism of Schistosoma mansoni miracidia.
The aerobic energy metabolism of Schistosoma mansoni miracidia.
复制标题
曼氏血吸虫的有氧能量代谢。
DOI：
10.1016/0166-6851(91)90211-n
复制
发表时间：
1991
影响因子：
1.5
通讯作者：
S. G. Bergh
中科院分区：
文献类型：
--
作者：
A. Tielens;Franciscus A.M. van de Pas;Josephus F.M. van den Heuvel;S. G. Bergh
什么时候。在[6-14C]葡萄糖存在下培养的曼氏酵母，70%的碳水化合物分解为二氧化碳，30%分解为乳酸。在3h的孵育过程中，平均23%的起始糖原储备被降解；外部葡萄糖的代谢不到总碳水化合物分解的2%。消耗的外部葡萄糖不到0.1%。在孵化过程中加入1毫升的小型˜M氰化物，完全消除了二氧化碳的产生，表明克雷布斯循环和呼吸链的活动是相连的。寡霉素严重抑制氧消耗。补充摘要：毛虫的能量代谢与尾蚴的能量代谢非常相似。像尾蚴一样，毛虫是依靠内源性糖原储备的崩溃而生存的自给自足的有机体，但在毛虫中，当糖原储备耗尽时，蛋白质可能会降解来补充这一点。毛滴虫的碳水化合物降解，如尾蚴，其特征是很大程度上依赖于Krebs循环活性，而Krebs循环活性与氧化磷酸化作用相耦合。即使在有氧条件下，毛虫和尾蚴也会产生大量的乳酸，这是呼吸链活动受到抑制时的唯一最终产物。
WhenS. mansonimiracidia were incubated in the presence of [6-14C]glucose, 70% of the carbohydrate breakdown resulted in the formation of CO2and 30% was broken down to lactate. During 3-h incubations, an average of 23% of the starting glycogen reserves were degraded; metabolism of external glucose accounted for less than 2% of the total carbohydrate breakdown. Less than 0.1% of the external glucose was consumed. Addition of 1 msmallcap˜M cyanide to the incubation completely abolished CO2production, demonstrating that the activities of the Krebs cycle and the respiratory chain are linked. Oligomycin severely inhibited oxygen consumption. It is concluded that the energy metabolism of miracidia strongly resembles that of cercariae.ADDITIONAL ABSTRACT:"The energy metabolism of miracidia strongly resembled that of cercariae, the other free-living stage ofS. mansoni.Like cercariae, miracidia are self-supporting organisms living on the breakdown of endogenous glycogen reserves, but in miracidia this is probably supplemented by protein degradation when the glycogen stores become exhausted. Carbohydrate degradation in miracidia, as in cercariae, is characterised by a large dependence on Krebs-cycle activity, which was coupled to oxidative phosphorylation. Even under aerobic conditions miracidia as well as cercariae also produced a significant amount of lactate, which was the exclusive end product when respiratory-chain activity was inhibited."