Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance
Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance
复制标题
DOI：
10.1016/j.bbrc.2011.05.080
复制
发表时间：
2011-06-17
影响因子：
3.1
通讯作者：
Koc, Ahmet
中科院分区：
文献类型：
--
作者：
Bozdag, Gonensin Ozan;Uluisik, Irem;Koc, Ahmet
硼是植物和动物必需的营养素，但过量会导致毒性。以前，Atr1和拟南芥Bor1同源物被鉴定为酵母中的硼外排泵，其降低细胞溶质硼浓度并帮助细胞在存在毒性量的硼的情况下存活。在这项研究中，我们分析了ATR 1旁系同源物YMR 279c和YOR 378w，以了解它们是否参与酵母的硼胁迫耐受性。尽管这些基因与ATR 1具有同源性，但它们的缺失既不会使细胞对硼敏感，也不会使硼处理显著上调它们的表达。然而，从组成型GAPDH启动子在高拷贝质粒上表达YMR 279，而不是YOR 378w，通过降低细胞内硼水平提供了显著的硼抗性。因此，我们的研究结果表明，存在第三个硼出口商，YMR 279c，其功能类似于ATR 1，并提供硼抗性酵母。(C)2011 Elsevier Inc. All rights reserved.
Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast. (C) 2011 Elsevier Inc. All rights reserved.