Probiotics Prevent Death Caused by Citrobacter rodentium Infection in Neonatal Mice
Probiotics Prevent Death Caused by Citrobacter rodentium Infection in Neonatal Mice
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DOI：
10.1086/648614
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发表时间：
2010-01-01
影响因子：
6.4
通讯作者：
Sherman, Philip M.
中科院分区：
文献类型：
--
作者：
Gareau, Melanie G.;Wine, Eytan;Sherman, Philip M.
背景肠道细菌感染是发病和死亡的主要原因，尤其是在新生儿中。因此，本研究的目的是描述新生小鼠中啮齿类柠檬酸杆菌感染的特征，并确定特定益生菌在改善疾病严重程度中所发挥的作用。用C.在出生后第14天进行啮齿动物试验。从第7天开始，用鼠李糖乳杆菌和瑞士乳杆菌的混合物或安慰剂口服预处理小鼠亚组。为了研究B和T细胞的作用，使用rag 1（-/-）和JH（-/-）小鼠，以存活率、结肠隐窝深度和屏障功能作为结果指标进行评价。C.啮齿类感染导致体重减轻和死亡。相反，通过每天用乳酸杆菌治疗来维持生存。体重减轻、结肠上皮细胞增生、粘膜屏障功能障碍和血清皮质酮水平升高。啮齿类感染的野生型小鼠被益生菌改善，但在rag 1（-/-）动物中没有。在B细胞缺陷（JH（-/-））小鼠中观察到益生菌的有益作用，表明T细胞在减少新生儿肠道感染的不良后遗症方面的需求。这些结果表明C.新生小鼠的啮齿类感染导致死亡，益生菌促进存活，但仅在T细胞存在的情况下。
Background. Intestinal bacterial infections are a major cause of morbidity and mortality, especially in neonates. Therefore, the aims of this study were to characterize Citrobacter rodentium infection in neonatal mice and determine the role played by specific probiotics in ameliorating disease severity.Methods. Infection of C57BL/6 mice with C. rodentium was performed at postnatal day 14. A subset of mice was pretreated orally with either a mixture of Lactobacillus rhamnosus and Lactobacillus helveticus or placebo, starting at 7 days. To study the effects of B and T cells, rag1(-/-) and JH(-/-) mice were used, with survival, colonic crypt depth, and barrier function evaluated as outcome measures.Results. C. rodentium infection caused weight loss and death. In contrast, survival was maintained by daily treatment with Lactobacilli. Weight loss, colonic epithelial cell hyperplasia, mucosal barrier dysfunction, and elevated serum corticosterone levels in C. rodentium-infected wild-type mice were ameliorated by probiotics, but not in rag1(-/-) animals. Beneficial effects of probiotics were observed in B cell-deficient (JH(-/-)) mice, indicating the requirement of T cells in reducing the adverse sequelae of neonatal enteric infection.Conclusions. These findings demonstrate that C. rodentium infection in newborn mice causes death and that probiotics promote survival, but only in the presence of T cells.