Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid
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DOI：
10.1183/09031936.03.00041703
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发表时间：
2003-10-01
影响因子：
24.3
通讯作者：
Gabrielsson, S
中科院分区：
文献类型：
--
作者：
Admyre, C;Grunewald, J;Gabrielsson, S
外泌体是通过内体区室的向内出芽形成的30-100 nm直径的囊泡，并且由几种细胞类型产生，包括T细胞、B细胞和树突状细胞（DC）。来自DC的外来体在其表面上表达主要组织相容性复合物（MHC）I类和II类以及共刺激分子，并且可以诱导T细胞的抗原特异性活化。本研究的目的是调查健康个体支气管肺泡灌洗液（BALF）中是否存在外泌体，并确定这些外泌体是否携带MHC和共刺激分子。作者分析了取自7名健康志愿者的BALF，并使用来自单核细胞来源的DC（MDDC）培养物的外泌体作为参考。超离心后，将外来体结合至抗MHC II类包被的磁珠，并通过流式细胞术和电子显微镜进行分析。作者首次报道了BALF中存在外泌体。这些外来体与MDDC来源的外来体相似，因为它们表达I类和II类MHC、CD 54、CD 63和共刺激分子CD 86。结果表明，外泌体存在于肺中，并且由于它们含有主要组织相容性复合物和共刺激分子，因此它们可能来源于抗原呈递细胞，并且可能在局部免疫防御中具有调节作用。
Exosomes are 30-100 nm diameter vesicles formed by inward budding of endosomal compartments and are produced by several cell types, including T-cells, B-cells and dendritic cells (DC)s. Exosomes from DCs express major histocompatibility complexes (MHC) class I and II, and co-stimulatory molecules on their surface, and can induce antigen-specific activation of T-cells. The aims of the present study were to investigate for the presence of exosomes in bronchoalveolar lavage fluid (BALF) from healthy individuals, and to establish if these exosomes bear MHC and co-stimulatory molecules. The authors analysed BALF taken from seven healthy volunteers and used exosomes from monocyte-derived DC (MDDC) cultures as a reference. After ultracentrifugation, exosomes were bound to anti-MHC class II coated magnetic beads and analysed by flow cytometry and electron microscopy. The authors report for the first time that exosomes are present in BALF. These exosomes are similar to MDDC derived exosomes as they express MHC class I and II, CD54, CD63 and the co-stimulatory molecule CD86. The results demonstrate that exosomes are present in the lung, and since they contain both major histocompatibility complex and co-stimulatory molecules it is likely that they are derived from antigen presenting cells and might have a regulatory role in local immune defence.