Hybrid identification of Cunninghamia lanceolata × Platycladus orientalis based on ISSR markers
Hybrid identification of Cunninghamia lanceolata × Platycladus orientalis based on ISSR markers
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发表时间：
2008
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通讯作者：
Qi Ming
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作者：
Qi Ming
杉木远缘杂种真实性鉴定利用从不列颠哥伦比亚省的100个引物组中筛选出的9个具有多态性的ISSR引物，对杉木×侧柏A4（1419）× B1远缘杂交组合进行了探索性试验，结果表明：选取4株表现优异的幼苗，通过DNA提取、9个多态性引物的PCR扩增、聚丙烯酰胺凝胶电泳（PAGE）对亲本和4株表现优异的幼苗进行分析，结果表明：（No.2，3，11，12）、父本和二溴化乙烯（EB）染色;用紫外灯观察DNA带型，用FR-200 A型生物电泳图像分析系统进行记录。结果表明，在A4 × B1组合中，9个多态性引物中有7个引物扩增出16条父本特异带，其余2个引物均未扩增出父本特异带，但在4个单株中均检测到双亲共有的一些条带，说明所选的优良单株均为远缘杂种，杉木×侧柏远缘杂交亲和性较好;杉木远缘杂交育种可用于林木多性状遗传改良。
To identify truthfulness of distant hybrids for Cunninghamia lanceolata(Chinese fir) × Platycladus orientalis,a series of exploratory experiments were conducted with 9 intersimple sequence repeat(ISSR) primers having polymorphism screened out from 100 University of British Columbia(UBC) primer Set # 9(800-900).For A4(1419) × B1 distant cross combinations of C.lanceolata × P.orientalis,four outstanding seedlings were selected.Analysis of the parents and the four outstanding seedlings was done with DNA extraction;polymerase chain reaction(PCR) amplification using 9 polymorphism primers;polyacrylamide gel electrophoresis(PAGE) based on the following order of maternal:A4,seedlings(No.2,3,11, 12),paternal,and ethylene dibromide(EB) staining;and DNA band observations with an ultraviolet lamp.Then,these were recorded with an FR-200A Bio-electrophoresis Image Analysis System.Experimental results of the four outstanding seedlings(No.2,3,11,12) showed that for the combination of A4 × B1:(1) there were 16 paternal special bands in 7 of the 9 polymorphic primers and(2) there were no paternal special bands with the other 2 primers,but some common bands of their parents were detected within the four seedlings.Thus,(1) all selected outstanding seedlings were distant hybrids;(2) distant hybridization between C.lanceolata × P.orientalis was compatible;and(3) wide cross breeding of Chinese fir could be used for multiple-trait genetic improvement of forest trees.