Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus
Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus
复制标题
甘蓝型油菜LAC基因家族的全基因组鉴定及其胁迫下的表达分析
DOI：
10.3390/molecules24101985
复制
发表时间：
2019-05-02
期刊：
影响因子：
4.6
通讯作者：
Liu, Liezhao
中科院分区：
文献类型：
--
作者：
Ping, Xiaoke;Wang, Tengyue;Liu, Liezhao
木质素是植物次生细胞壁发育所必需的重要生物聚合物。漆酶（LAC）基因家族催化木质化，在植物界起着重要作用。在本研究中，我们从甘蓝型油菜基因组中鉴定了45个LAC基因（BnLACs），从芜菁基因组中鉴定了25个LAC基因（BrLACs），从甘蓝基因组中鉴定了8个LAC基因（BoLACs）。这些LAC基因在一个进化树中可分为5个类群，同一类群中的成员具有相似的结构和保守的基序。所有的BnLAC含有激素和应力相关的元素确定的顺式元件分析。BnLACs在根、种皮和茎中的表达量相对较高，而在其它组织中的表达量相对较低。此外，BnLAC 4及其预测的下游基因表现出较早的表达在角果果皮短角果系比长角果系。还预测了三种miRNA（miR 397 a、miR 397 b和miR 6034）靶向11种BnLAC。通过RNA测序（RNA-seq）和实时定量聚合酶链反应（qRT-PCR）进一步研究BnLACs在一系列胁迫下的表达变化。本研究为进一步了解LAC基因家族在B中的进化和功能奠定了基础。油菜。
Lignin is an important biological polymer in plants that is necessary for plant secondary cell wall ontogenesis. The laccase (LAC) gene family catalyzes lignification and has been suggested to play a vital role in the plant kingdom. In this study, we identified 45 LAC genes from the Brassica napus genome (BnLACs), 25 LAC genes from the Brassica rapa genome (BrLACs) and 8 LAC genes from the Brassica oleracea genome (BoLACs). These LAC genes could be divided into five groups in a cladogram and members in same group had similar structures and conserved motifs. All BnLACs contained hormone- and stress- related elements determined by cis-element analysis. The expression of BnLACs was relatively higher in the root, seed coat and stem than in other tissues. Furthermore, BnLAC4 and its predicted downstream genes showed earlier expression in the silique pericarps of short silique lines than long silique lines. Three miRNAs (miR397a, miR397b and miR6034) target 11 BnLACs were also predicted. The expression changes of BnLACs under series of stresses were further investigated by RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT-PCR). The study will give a deeper understanding of the LAC gene family evolution and functions in B. napus.