Polypyrrole nanotube array sensor for enhanced adsorption of glucose oxidase in glucose biosensors
Polypyrrole nanotube array sensor for enhanced adsorption of glucose oxidase in glucose biosensors
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DOI：
10.1016/j.bios.2007.03.022
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发表时间：
2007-08-30
影响因子：
12.6
通讯作者：
Kaneto, Keiichi
中科院分区：
文献类型：
--
作者：
Ekanayake, E. M. I. Mala;Preethichandra, D. M. G.;Kaneto, Keiichi
报道了一种基于纳米多孔氧化铝基底上沉积的聚吡咯（PPy）纳米管阵列的电流型生物传感器及其性能。以葡萄糖氧化酶（GOx）为模型酶。利用市售的纳米多孔氧化铝圆片制作电极，研究物理吸附法包埋酶的可行性。在0.3mA/cm（2）的电流密度下，使用含有0.05M吡咯和0.1M NaPF 6的溶液90秒来合成包含纳米管阵列的PPy/PF 6-膜。通过在每个电极上物理吸附5 μ L GOx（来自2 mg/ML的210 U/mg的储备溶液）来进行固定。最大管径为100 nm的聚吡咯纳米管阵列的灵敏度为7.4 mA cm（-2）M-1。用最大管直径为200 nm的纳米管阵列观察到500 μ M-13 mM的线性范围和约3 s的响应时间。采用恒电流电化学方法对所合成的纳米管阵列进行了表征。表观米氏常数（K-m）的计算值为7.01 mM。使用纳米多孔模板电极导致有效的酶负载，并提供用于感测反应的增加的表面积。这些因素有助于提高新型生物传感器的特性性能。(C)2007 Elsevier B. V.保留所有权利。
A novel amperometric biosensor based on polypyrrole (PPy) nanotube array deposited on a Pt plated nano-porous alumina substrate and its performances are described. Glucose oxidase (GOx) enzyme was selected as the model enzyme in this study. Commercially available nano-porous alumina discs were used to fabricate electrodes in order to study the feasibility of enzyme entrapment by physical adsorption. A PPy/PF6- film comprising of nanotube array was synthesized using a solution containing 0.05 M Pyrrole and 0.1 M NaPF6 at a current density of 0.3 mA/cm(2) for 90s. The immobilization was done by physical adsorption of 5 mu L of GOx (from a stock solution of 2 mg/ML of 210 U/mg) on each electrode. A sensitivity of 7.4 mA cm(-2) M-1 was observed with PPy nanotube array where the maximum tube diameter was 100 nm. A linear range of 500 mu M-13 mM and a response time of about 3 s were observed with a nanotube array where the maximum tube diameter was 200 nm. The synthesized nanotube arrays were characterized by galvanostatic electrochemical technique. Calculated value of apparent Michaelis-Menten constant (K-m) was 7.01 mM. The use of nano-porous template electrodes leads to an efficient enzyme loading and provides an increased surface area for sensing the reaction. These factors contribute to increase the characteristic performances of the novel biosensor. (C) 2007 Elsevier B.V. All rights reserved.