In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities
In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities
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通过在具有不同电荷密度的合成水凝胶上培养来增强软骨形成 ATDC5 细胞的体外分化
DOI：
10.1016/j.actbio.2009.07.033
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发表时间：
2010-02-01
影响因子：
9.7
通讯作者：
Gong, Jian Ping
中科院分区：
文献类型：
--
作者：
Kwon, Hyuck Joon;Yasuda, Kazunori;Gong, Jian Ping
我们研究了不同电荷密度的合成聚合物凝胶：带负电荷的聚(2-丙烯酰胺-2-甲基-1-丙磺酸)(PAMPS)凝胶、中性聚二甲基丙烯酰胺(PDMAAm)凝胶以及不同组成(AMPS的摩尔分数，F=0.25、0.5、0.75)的2-丙烯酰胺-2-甲基-1-丙磺酸与二甲基丙烯酰胺P(AMPS-co-DMAAm)的共聚凝胶(AMPS-co-DMAAm)在ATDC5细胞上的行为。在无胰岛素维持液中，ATDC5细胞在高负电荷凝胶-PAMPS凝胶和P(AMPS-co-DMAAm)共聚凝胶(F=0.75)上培养，第7天铺展并融合，第14天有趣地形成结节，表达11型胶原和蛋白多糖。这一结果表明，即使在无胰岛素的维持介质中，在标准的聚苯乙烯培养皿中培养的ATDC5细胞不能分化为软骨细胞，高负电荷的凝胶也可以诱导ATDC5细胞向软骨细胞分化。在含胰岛素的分化培养液中，在PDMAAm凝胶上培养的ATDC5细胞在7d内形成局灶性粘连，迅速聚集并形成大的结节，其11型胶原和蛋白多糖的表达水平明显高于在聚苯乙烯培养皿和带负电的凝胶上培养的细胞。结果表明，中性凝胶可促进ATDC5细胞在分化介质中向软骨细胞分化。我们认为，高负电荷的PAMPS凝胶和中性的PDMAAM凝胶是一种有潜力的软骨组织工程支架材料，具有诱导成软骨细胞分化的潜力。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
We investigated the behavior of chondrogenic ATDC5 cells on synthetic polymer gels with various charge densities: negatively charged poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS) gel, neutral poly(dimethylacrylamide) (PDMAAm) gel, and copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid and dimethylacrylamide P(AMPS-co-DMAAm) with different compositions (molar fractions of AMPS, F = 0.25, 0.5, 0.75). In insulin-free maintenance medium, the ATDC5 cells cultured on the highly negatively charged gels - PAMPS gel and the P(AMPS-co-DMAAm) copolymer gels (F = 0.75) - spread and became confluent at day 7, and interestingly formed nodules at day 14, expressing type 11 collagen and proteoglycan. This result demonstrates that the highly negatively charged gels can induce chondrogenic differentiation of ATDC5 cells even in insulin-free maintenance medium, in which the ATDC5 cells cultured on the standard polystyrene dish cannot differentiate into chondrocytes. In insulin-supplemented differentiation medium, ATDC5 cells cultured on the PDMAAm gel made focal adhesions, rapidly aggregated and formed large nodules within 7 days, expressing significantly greater levels of type 11 collagen and proteoglycan than cells cultured on the polystyrene dish and the negatively charged gels. These results showed that the neutral gel accelerated chondrogenic differentiation of ATDC5 cells cultured in the differentiation medium. We suggest that the highly negatively charged PAMPS gel and the neutral PDMAAm gel are interesting biomaterials for cartilage tissue engineering as a scaffold with the potential to induce chondrogenic differentiation. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.