Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2
Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2
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DOI：
10.1016/j.febslet.2012.06.008
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发表时间：
2012-07-30
期刊：
影响因子：
3.5
通讯作者：
Ohsumi, Yoshinori
中科院分区：
文献类型：
--
作者：
Kobayashi, Takafumi;Suzuki, Kuninori;Ohsumi, Yoshinori
Atg 2-Atg 18复合物是酿酒酵母中自噬体形成所必需的。在本文中，我们表明，自噬的部分诱导可以在细胞中进行，表达工程化的变体Atg 2能够本地化的前自噬体结构（PAS）在Atg 18的情况下。具体而言，通过构建融合蛋白，我们表明，无论是磷脂酰肌醇3-磷酸结合FYVE域或核心自噬蛋白Atg 8的Atg 2融合允许有限的Atg 18独立的自噬体形成的恢复。这些结果表明，Atg 2有效靶向PAS可以补偿Atg 18在自噬中功能的丧失。(c)2012年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The Atg2-Atg18 complex is essential for autophagosome formation in Saccharomyces cerevisiae. In this paper, we show that partial induction of autophagy can proceed in cells expressing engineered variants of Atg2 capable of localizing to the pre-autophagosomal structure (PAS) in the absence of Atg18. Specifically, through the construction of fusion proteins, we show that the fusion to Atg2 of either the phosphatidylinositol 3-phosphate-binding FYVE domain or the core autophagy protein Atg8 allowed limited Atg18-independent recovery of autophagosome formation. These results indicate that effective targeting of Atg2 to the PAS can compensate for loss of Atg18 function in autophagy. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.