Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50% PNT).
Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50% PNT).
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检测%20of%20rainbow%20trout%20抗体%20to%20Egtved%20病毒%20by%20酶联%20免疫吸附剂%20检测%20(ELISA),%20免疫荧光%20(IF),%20和%20斑块%20中和%20测试%20(
DOI：
10.3354/dao010031
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发表时间：
1991
影响因子：
1.4
通讯作者：
P. Jørgensen
中科院分区：
文献类型：
--
作者：
N. J. Olesen;N. Lorenzen;P. Jørgensen
通过酶联免疫吸附测定 (ELISA)、免疫荧光 (IF) 和 50'X 空斑中和试验 (50°/"PNT) 研究了 Egtved 病毒（虹鳟鱼虹鳟病毒性出血性败血症 (VHS) 的病原体）的体液免疫反应。在水族箱条件下用病毒免疫的鱼的血清以及从鱼中收集的血清在实验中，ELISA 被证明比 IF 或 50°/~PNT 更灵敏，且耗时更少。使用 Elisa，在来自受感染鳟鱼养殖场的 54% 的鱼中发现了 Egtved 病毒抗体，而 50°/PNT 检测中只有 16% 呈阳性。 IF 测试的灵敏度接近 ELISA，但不太适合检查大量鱼。非中和抗体在 VHS 感染后往往比中和抗体持续时间更长。我们的结论是，我们的 ELISA 非常适合 VHS 监测。
The humoral immune response to Egtved virus, the causative agent of viral haemorrhagic septicaemia (VHS) in rainbow trout Oncorhynchus mykiss, was investigated by means of the enzymelinked immunosorbent assay (ELISA), immunofluorescence (IF), and the 50 'X plaque neutralization test (50°/"PNT). Sera from fish immunized with virus under aquarium conditions as well a s sera collected from fish in farms with different VHS status were included In the experiments. ELISA proved to be more sensitive and less timeand material-consuming than either IF or 50°/~PNT Using Elisa, antibody to Egtved virus was found in 54 %I of fish from infected trout farms examined w h ~ l e only 16 % were positive by 50°/r,PNT The IF test had a sensitivity close to that of ELISA, but was less suitable for examination of large numbers of sera. Non-neutralizing antibodies tend to perslst longer after VHS infection than neutralizing antibodies. The kinetics of the antibody response was close to that prev~ously published. No anamnestic immune response was observed with any of the tests. We conclude that our ELISA is well suited for VHS surveillance.