Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae.
Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae.
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DOI：
10.1016/j.marpolbul.2011.01.022
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发表时间：
2011
影响因子：
5.8
通讯作者：
Masahumi Kawaguchi;Jun-Young Song;K. Irie;Y. Murakami;K. Nakayama;S. Kitamura
中科院分区：
文献类型：
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作者：
Masahumi Kawaguchi;Jun-Young Song;K. Irie;Y. Murakami;K. Nakayama;S. Kitamura
众所周知，石油泄漏会给水生生物带来严重的问题。特别是一些种类的硬骨鱼，它们生长在海面上，被认为受到重油(HO)的影响。在胚胎发育过程中，神经系统被构建起来。因此，研究HO对发育中神经元的毒理作用具有重要意义。我们将HO暴露于牙鲆(Paralichthys Olivaceus)的卵中，并研究了其神经功能障碍。在浓度为8.75 mg/L的HO染毒的幼虫中，面神经和侧线神经部分进入错误区域，束脱束。此外，在HO暴露的幼虫中，一种轴突引导分子Sema3A在面神经的靶区第二咽弓广泛表达。综上所述，我们认为可能是HO暴露影响了Sema3A的异常表达，导致面神经支架的破坏。
It has been well known that oil spills cause serious problems in the aquatic organisms. In particular, some species of teleosts, which develop on the sea surface thought to be affected by heavy oil (HO). During the embryogenesis, the nervous system is constructed. Therefore, it is important to study the toxicological effects of HO on the developing neurons. We exposed HO to eggs of Japanese flounder (Paralichthys olivaceus) and investigated the neural disorder. In larvae exposed by HO at the concentration of 8.75mg/L, the facial and lateral line nerves partially entered into the incorrect region and the bundle was defasciculated. Furthermore, in the HO-exposed larvae, Sema3A, a kind of axon guidance molecule, was broadly expressed in second pharyngeal arch, a target region of facial nerve. Taken together, we suggested the possibility that the abnormal expression of Sema3A affected by HO exposure causes disruption of facial nerve scaffolding.