Stimulation of mono- and diacylglycerol lipase activities by gangliosides in chicken neuronal cultures.
Stimulation of mono- and diacylglycerol lipase activities by gangliosides in chicken neuronal cultures.
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鸡神经元培养物中神经节苷脂对单酰基甘油和二酰基甘油脂肪酶活性的刺激。
DOI：
10.1007/bf00966702
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发表时间：
1991
影响因子：
4.4
通讯作者：
Dreyfus,H
中科院分区：
文献类型：
--
作者：
Freysz,L;Farooqui,AA;Horrocks,LA;Massarelli,R;Dreyfus,H
培养的鸡神经元显示出可测量的单甘油和双甘油脂肪酶的活性。神经节苷脂(10−8M~10−5M)处理鸡神经细胞后，单甘油脂肪酶活性呈时间和剂量依赖性增加。双甘油脂肪酶的比活力明显高于单甘油脂肪酶。在48h后，对照组和神经节苷脂处理组细胞的二酰甘油脂肪酶活性没有差异。这些数据表明，外源性神经节苷脂处理神经元，不仅能刺激催化其产生的酶，而且还能刺激参与其分解代谢的酶，从而影响双甘油酯的代谢。
Chicken neurons in culture display measurable activities of mono-and diacylglycerol lipases. Treatment of chicken neuronal cultures with gangliosides (10−8M to 10−5M) resulted in a time and dose dependent increase in monoacylglycerol lipase activity. The diacylglycerol lipase showed significant increase in specific activity before that of monoacylglycerol lipase. The increase was observed only up to 24 hours and no differences between diacylglycerol lipase activity of control and ganglioside treated cells were observed after 48 hours. The data indicate that the treatment of neurons with exogenous gangliosides affect the diglyceride metabolism in stimulating not only the enzymes catalyzing their production but also those involved in their catabolism.