Bone marrow-derived mesenchymal stromal cells differ in their attachment to fibronectin-derived peptides from term placenta-derived mesenchymal stromal cells.
Bone marrow-derived mesenchymal stromal cells differ in their attachment to fibronectin-derived peptides from term placenta-derived mesenchymal stromal cells.
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DOI：
10.1186/s13287-015-0243-6
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发表时间：
2016-02-11
影响因子：
7.5
通讯作者：
Aicher WK
中科院分区：
文献类型：
--
作者：
Maerz JK;Roncoroni LP;Goldeck D;Abruzzese T;Kalbacher H;Rolauffs B;DeZwart P;Nieselt K;Hart ML;Klein G;Aicher WK
人骨髓间充质干细胞（mesenchymal stromal cells，MSCs）可从不同来源分离，包括骨髓和胎盘。这两个群体在体外显示出不同的增殖和分化模式。由于细胞的增殖和分化受到细胞-基质相互作用的调节，我们研究了MSC与一组肽包被表面的附着，并探索了它们与悬浮液中肽的相互作用。从骨髓和足月胎盘中分离人MSC并扩增。通过细胞附着点测定、阻断实验和流式细胞术研究MSC与肽的结合。整合素的表达模式进行了探讨，通过转录阵列和定量逆转录聚合酶链反应和流式细胞术证实。扩增的胎盘来源的MSC（pMSC）很好地附着于用纤连蛋白来源的肽P7、P15和P17包被的表面，而骨髓来源的MSC（bmMSC）附着于P7，但几乎不附着于P15和P17。β1整合素介导bmMSCs和pMSCs与肽的结合。在悬浮液中，扩增的bmMSC几乎不与P7、P13、P15结合，并且较少与P14和P17结合。离体，bmMSCs未能结合P7，但显示与P13，P14和P15的弱相互作用。在悬浮液中，扩增的pMSC显示与许多肽结合，包括P4、P7、P13、P14、P15和P17。观察到的bmMSC和pMSC与肽结合的差异与整合素α2-、α4-和α6-链表达的显著差异相关。人bmMSC和pMSC显示出与限定肽的不同附着模式，并在体外维持整合素表达的差异。与悬浮液中扩增的bmMSC相比，离体bmMSC与给定肽的相互作用产生不同的染色模式。扩增的MSC与表面上的肽的附着不同于扩增的MSC与悬浮液中的肽的相互作用。因此，设计用于研究人MSC与肽增强支架或悬浮肽的相互作用的研究必须考虑细胞-肽相互作用的这些差异。本文的在线版本（doi：10.1186/s13287-015-0243-6）包含补充材料，可供授权用户使用。
Human mesenchymal stromal cells (MSCs) can be isolated from different sources including bone marrow and term placenta. These two populations display distinct patterns of proliferation and differentiation in vitro. Since proliferation and differentiation of cells are modulated by cell–matrix interactions, we investigated the attachment of MSCs to a set of peptide-coated surfaces and explored their interactions with peptides in suspension. Human MSCs were isolated from bone marrow and term placenta and expanded. Binding of MSCs to peptides was investigated by a cell-attachment spot assay, by blocking experiments and flow cytometry. The integrin expression pattern was explored by a transcript array and corroborated by quantitative reverse transcription polymerase chain reaction and flow cytometry. Expanded placenta-derived MSCs (pMSCs) attached well to surfaces coated with fibronectin-derived peptides P7, P15, and P17, whereas bone marrow-derived MSCs (bmMSCs) attached to P7, but barely to P15 and P17. The binding of bmMSCs and pMSCs to the peptides was mediated by β1 integrins. In suspension, expanded bmMSCs barely bind to P7, P13, P15, and less to P14 and P17. Ex vivo, bmMSCs failed to bind P7, but displayed a weak interaction with P13, P14, and P15. In suspension, expanded pMSCs displayed binding to many peptides, including P4, P7, P13, P14, P15, and P17. The differences observed in binding of bmMSCs and pMSCs to the peptides were associated with significant differences in expression of integrin α2-, α4-, and α6-chains. Human bmMSCs and pMSCs show distinct patterns of attachment to defined peptides and maintain differences in expression of integrins in vitro. Interactions of ex vivo bmMSCs with a given peptide yield different staining patterns compared to expanded bmMSCs in suspension. Attachment of expanded MSCs to peptides on surfaces is different from interactions of expanded MSCs with peptides in suspension. Studies designed to investigate the interactions of human MSCs with peptide-augmented scaffolds or peptides in suspension must therefore regard these differences in cell–peptide interactions. The online version of this article (doi:10.1186/s13287-015-0243-6) contains supplementary material, which is available to authorized users.