Production of the antibacterial substance by Bacillus sp. strain NM 12, an intestinal bacterium of Japanese coastal fish
Production of the antibacterial substance by Bacillus sp. strain NM 12, an intestinal bacterium of Japanese coastal fish
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DOI：
10.1016/s0044-8486(98)00267-1
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发表时间：
1998-06-15
期刊：
影响因子：
4.5
通讯作者：
Deguchi, Y
中科院分区：
文献类型：
--
作者：
Sugita, H;Hirose, Y;Deguchi, Y
用双层法检测了从7种近海鱼类中分离的肠道细菌对创伤弧菌RIMD-2219009的抑菌作用。在检测的1055株细菌中，有28株(2.7%)能抑制目标菌株的生长。高活性(大于或等于20.0 mm)只有15株(1.4%)，包括11株弧菌科、3株棒状杆菌和1株芽孢杆菌。从蜻蜓肠道分离到的菌株NM-12显示出最高的活性，被选为活性菌株。该菌在20℃、25%海水配制的50%PYBG液体培养基(pH=6.5)中培养48h，能有效地产生抗菌物质。其中至少有一种抗菌物质是分子质量小于5 kDa的不耐热铁载体，具有广泛的抗菌谱：它对7种近海鱼类363株肠道细菌中的227株(62.5%)有抑制作用。这些结果表明，芽孢杆菌属。菌株NM12可作为鱼肠道和养殖水体的生防菌。(C)1998 Elsevier Science B.V.保留所有权利。
Intestinal bacteria isolated from seven coastal fish were examined for their antibacterial ability against Vibrio vulnificus RIMD 2219009 using a double-layer method. Of 1055 bacteria examined, 28 (2.7%) isolates could inhibit the growth of the target strain. High activities (greater than or equal to 20.0 mm) were only seen in 15 isolates (1.4%), which were composed of 11 Vibrionaceae, three coryneforms and one Bacillus isolates, Since Bacillus sp. strain NM 12 isolated from the intestine of a dragonets (Callionymus sp.) showed the highest activity, it was chosen as an active strain. This bacterium efficiently produced an antibacterial substance when incubated in 50% PYBG liquid medium (pH = 6.5) prepared with 25% seawater for 48 h at 20 degrees C. At least one of the antibacterial substances was a heat labile siderophore with a molecular mass of less than 5 kDa and had a wide antibacterial spectrum: it inhibited the growth of 227 (62.5%) of 363 intestinal bacteria from seven coastal fish. These results suggest that Bacillus sp. strain NM 12 may be a suitable strain as a biocontrol agent in fish intestines and culture water. (C) 1998 Elsevier Science B.V. All rights reserved.