ELECTROPHYSIOLOGIC AND MECHANICAL EFFECTS OF METABOLIC INHIBITION OF HIGH-ENERGY PHOSPHATE PRODUCTION IN CULTURED CHICK-EMBRYO VENTRICULAR CELLS
ELECTROPHYSIOLOGIC AND MECHANICAL EFFECTS OF METABOLIC INHIBITION OF HIGH-ENERGY PHOSPHATE PRODUCTION IN CULTURED CHICK-EMBRYO VENTRICULAR CELLS
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DOI：
10.1016/s0022-2828(84)80014-0
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发表时间：
1984-01-01
影响因子：
5
通讯作者：
BARRY, WH
中科院分区：
文献类型：
--
作者：
HASIN, Y;DOOREY, A;BARRY, WH
在培养的鸡胚心肌细胞上研究了代谢抑制高能磷酸盐产生的早期电生理和机械效应。选择性抑制糖酵解的2-脱氧葡萄糖在乙酸或氧化磷酸化CN的存在下表现出不同的效果。2-脱氧葡萄糖诱导最大舒张电位显著降低，兴奋-收缩延迟延长，收缩力仅中度降低，动作电位时程变化极小。CN诱导了深刻的负性肌力作用，并引起松弛减慢，动作电位时程缩短，动作电位上行减少，舒张膜电位仅中度降低。暴露于2-脱氧葡萄糖和CN组合产生的影响与抑制两种代谢途径一致。这些观察结果与这两种代谢途径可能在为心肌细胞的几种能量需求功能提供燃料方面具有特定作用的假设一致。
The early electrophysiological and mechanical effects of metabolic inhibition of high energy phosphate production were studied in cultured chick embryo heart cells. Selective inhibition of either glycolysis by 2-deoxyglucose in the presence of acetate or of oxidative phosphorylation by CN showed different effects. 2-Deoxyglucose induced pronounced reduction in maximal diastolic potential and prolongation of excitation-contraction delay, with only a moderate decrease of contractility and with only minimal changes in action potential duration. CN induced a profound negative inotropic effect and caused slowing of relaxation, shortening of action potential duration, a decrease in the upstroke of the action potential and only a moderate decrease in the diastolic membrane potential. Exposure to 2-deoxyglucose and CN combined produced effects consistent with inhibition of both metabolite pathways. These observations are consistent with the hypothesis that these 2 metabolic pathways may have specific roles in fueling several energy-demanding functions of the myocardial cell.