Expression Signatures of Long Noncoding RNAs in Adolescent Idiopathic Scoliosis.
Expression Signatures of Long Noncoding RNAs in Adolescent Idiopathic Scoliosis.
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青少年特发性脊柱侧弯中长非编码 RNA 的表达特征
DOI：
10.1155/2015/276049
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发表时间：
2015
影响因子：
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通讯作者：
Wang YP
中科院分区：
文献类型：
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作者：
Liu XY;Wang L;Yu B;Zhuang QY;Wang YP
目的。青少年特发性脊柱侧凸(AIS)是最常见的儿童脊柱畸形，被认为是一种复杂的遗传病。AIS的致病基因和发病机制尚不清楚。本研究旨在鉴定与AIS发病相关的差异表达的长非编码RNA(LncRNAs)。方法：研究方法。我们首先使用安捷伦人类LncRNA+mRNA阵列V3.0微阵列对AIS患者和健康儿童进行了LncRNA和mRNA的全面筛选。用定量聚合酶链式反应进一步检测不同类型AIS患者中LncRNAs的表达。结果。在AIS患者和健康对照组中，共有139个LncRNAs和546mRNAs差异表达。GO和Path分析表明，这些mRNAs可能参与了骨矿化、神经肌肉连接、骨骼系统的形态发生、核苷酸和核酸代谢以及信号通路的调节。根据年龄、身高、分类、脊柱侧弯严重程度和Risser分级分组，4个LncRNAs(ENST00000440778.1、ENST00000602322.1、ENST00000414894.1和TCONS_00028768)在不同的患者中有不同的表达。结论。本研究证实了lncRNAs和mRNAs在AIS中的异常表达，部分lncRNAs的表达与AIS的临床特征有关。本研究有助于进一步了解lncRNAs在AIS发病机制、治疗及预后中的作用。
Purpose. Adolescent idiopathic scoliosis (AIS), the most common pediatric spinal deformity, is considered a complex genetic disease. Causing genes and pathogenesis of AIS are still unclear. This study was designed to identify differentially expressed long noncoding RNAs (lncRNAs) involving the pathogenesis of AIS. Methods. We first performed comprehensive screening of lncRNA and mRNA in AIS patients and healthy children using Agilent human lncRNA + mRNA Array V3.0 microarray. LncRNAs expression in different AIS patients was further evaluated using quantitative PCR. Results. A total of 139 lncRNAs and 546 mRNAs were differentially expressed between AIS patients and healthy control. GO and Pathway analysis showed that these mRNAs might be involved in bone mineralization, neuromuscular junction, skeletal system morphogenesis, nucleotide and nucleic acid metabolism, and regulation of signal pathway. Four lncRNAs (ENST00000440778.1, ENST00000602322.1, ENST00000414894.1, and TCONS_00028768) were differentially expressed between different patients when grouped according to age, height, classification, severity of scoliosis, and Risser grade. Conclusions. This study demonstrates the abnormal expression of lncRNAs and mRNAs in AIS, and the expression of some lncRNAs was related to clinical features. This study is helpful for further understanding of lncRNAs in pathogenesis, treatment, and prognosis of AIS.