Designing the folding mechanics of coiled coils.
Designing the folding mechanics of coiled coils.
复制标题
设计卷绕线圈的折叠机制。
DOI：
10.1002/cphc.200900575
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
M. Rief
中科院分区：
文献类型：
--
作者：
T. Bornschlögl;J. Christof M. Gebhardt;M. Rief
自然形成的盘绕线圈在整个结构中往往不是均匀的，而是被序列不连续和非盘绕线圈形成的亚段打断。我们应用原子力显微镜局部探测了当加入不同大小的非结构子段时，一个众所周知的模型线圈的机械折叠/展开过程。我们发现，随着增加的非结构子段的尺寸增加，反折叠力从7.8pN减小，而模型盘绕线圈的展开特性保持不变。我们表明，这一行为是由于成核种子的尺寸增加，在进一步的卷曲折叠可以进行之前必须形成成核种子。由于成核种的大小与高能折叠势垒的宽度有关，因此我们能够直接测量折叠力与势垒宽度的关系。我们的结果允许通过简单地调整成核种子大小来设计具有指定折叠力的卷绕线圈。
Naturally occurring coiled coils are often not homogeneous throughout their entire structure but rather interrupted by sequence discontinuities and non-coiled-coil-forming subsegments. We apply atomic force microscopy to locally probe the mechanical folding/unfolding process of a well-understood model coiled coil when unstructured subsegments with different sizes are added. We find that the refolding force decreases from 7.8 pN with increasing size of the added unstructured subsegment, while the unfolding properties of the model coiled coil remain unchanged. We show that this behavior results from the increased size of the nucleation seed which has to form before further coiled-coil folding can proceed. Since the nucleation seed size is linked to the width of the energetic folding barrier, we are able to directly measure the dependence of folding forces on the barrier width. Our results allow the design of coiled coils with designated refolding forces by simply adjusting the nucleation seed size.
登录
查看更多内容
影响因子：
3
作者：
Chana, M;Tripet, BP;Hodges, RS
通讯作者：
Hodges, RS
影响因子：
3.4
作者：
Evans, E;Ritchie, K
通讯作者：
Ritchie, K
影响因子：
5.6
作者：
J. Zitzewitz;Beatriz Ibarra-Molero;Deanna R Fishel;Kimberly L Terry;C. Matthews
通讯作者：
J. Zitzewitz;Beatriz Ibarra-Molero;Deanna R Fishel;Kimberly L Terry;C. Matthews
DOI：
10.1073/pnas.96.19.10699
发表时间：
1999-09
影响因子：
11.1
作者：
Liam B. Moran;Joel P. Schneider;A. Kentsis;Giridher A. Reddy;T. Sosnick
通讯作者：
Liam B. Moran;Joel P. Schneider;A. Kentsis;Giridher A. Reddy;T. Sosnick
影响因子：
56.9
作者：
OSHEA, EK;KLEMM, JD;ALBER, T
通讯作者：
ALBER, T