Reelin, Disabled 1, and β1 integrins are required for the formation of the radial glial scaffold in the hippocampus
Reelin, Disabled 1, and β1 integrins are required for the formation of the radial glial scaffold in the hippocampus
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DOI：
10.1073/pnas.202035899
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发表时间：
2002-10-01
影响因子：
11.1
通讯作者：
Frotscher, M
中科院分区：
文献类型：
--
作者：
Förster, E;Tielsch, A;Frotscher, M
细胞外基质分子Reelin是前脑发育过程中神经元正确定位所必需的。然而，Reelin对神经元迁移的作用机制知之甚少。Reelin被认为直接作用于神经元，但它也可能影响神经元迁移所必需的胶质细胞的分化。在这里，我们表明，一个规则的胶质细胞支架未能形成在体内的小鼠的齿状回缺乏Reelin或残疾1，神经元衔接蛋白的Reelin信号通路。这些缺陷的一个子集是在缺乏β（1）类整合素的小鼠中观察到的，已知整合素与Reelin结合。此外，重组Reelin在体外诱导神经胶质过程的分支。我们的数据表明，Reelin通过Disabled 1和β（1）类整合素依赖性信号通路影响胶质细胞分化。
The extracellular matrix molecule Reelin is required for the correct positioning of neurons during the development of the forebrain. However, the mechanism of Reelin action on neuronal migration is poorly understood. Reelin is assumed to act on neurons directly, but it may also affect the differentiation of glial cells necessary for neuronal migration. Here we show that a regular glial scaffold fails to form in vivo in the dentate gyrus of mice deficient of Reelin or Disabled 1, a neuronal adaptor protein in the Reelin signaling pathway. A subset of these defects is observed in mice that lack beta(1)-class integrins, known to bind Reelin. Moreover, recombinant Reelin induced branching of glial processes in vitro. Our data suggest that Reelin affects glial differentiation via Disabled 1 and beta(1)-class integrin-dependent signaling pathways.