E-cadherin, CD44 and CD44v6 in squamous intraepithelial lesions and invasive carcinomas of the uterine cervix: An immunohistochemical study
E-cadherin, CD44 and CD44v6 in squamous intraepithelial lesions and invasive carcinomas of the uterine cervix: An immunohistochemical study
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DOI：
10.1159/000081729
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发表时间：
2004-01-01
期刊：
影响因子：
5
通讯作者：
Lopes, C
中科院分区：
文献类型：
--
作者：
Faleiro-Rodrigues, C;Lopes, C
目的：目的探讨E-cadherin、CD 44 std和v6在宫颈正常鳞状上皮、低、高鳞状上皮内病变（锡尔斯）和浸润性鳞状细胞癌（ISCC）中的表达。目的是确定抗原表达的任何独特变化是否有助于识别最早的肿瘤形成和/或侵袭性表型的发生。研究方法：应用免疫组织化学方法检测了124例宫颈标本（5例正常、39例低度恶性、54例高度恶性和26例ISCCS）中上皮钙粘蛋白（E-cadherin）、簇分化蛋白44（CD 44）和亚型v6（CD 44 v6）的蛋白表达。结果如下：在正常鳞状上皮和低度鳞状上皮内病变中，E-cadherin、CD 44和CD 44 v6的膜表达得以保留。观察到锡尔斯的组织学分级和E-cadherin（p < 0.001）、CD 44 std（p = 0.027）和CD 44 v6（p < 0.001）的免疫反应性（膜型与胞浆型）模式显著相关。从低级别到高级别的锡尔斯到ISCC，观察到膜染色的损失和细胞质染色的逐渐增加。结论：我们的研究表明，在宫颈病变的发展过程中，大量的定性（亚细胞定位膜到细胞质）和定量的变化（表达的变化）发生在宫颈癌中的E-钙粘蛋白，CD 44和CD 44 v6的蛋白表达。最引人注目的观察是减少膜免疫反应性和逐步增加的细胞质染色的E-钙粘蛋白，CD 44和CD 44 v6，有关的损失分化的结果肿瘤转化。版权所有（C）2004 S. Karger AG，巴塞尔。
Objective: To evaluate the immunoexpression pattern of E-cadherin, CD44std and the variant isoform v6 in normal squamous epithelium, low and high squamous intraepithelial lesions (SILs) and invasive squamous cell carcinomas (ISCCs) of the uterine cervix. The purpose was to determine whether any distinctive change in antigenic expression could contribute to the recognition of the earliest commitment to neoplasia and/or the onset of the invasive phenotype. Methods: Immunohistochemistry using the avidin- biotin indirect immunoperoxidase method was used to study the protein expression of epithelial cadherin (E-cadherin), cluster differentiation 44 (CD44), and the isoform v6 (CD44v6) in 124 human cervical samples ( 5 normal, 39 low-grade, 54 high-grade and 26 ISCCS) in formalin-fixed, paraffin-embedded tissue blocks. Results: Membranous expression of E-cadherin, CD44 and CD44v6 was preserved in normal squamous epithelium and in low-grade squamous intraepithelial lesions. A significant association was observed with the histological grade of the SILs and the immunoreactivity ( membranous versus cytoplasmic) pattern of E-cadherin ( p < 0.001), CD44std ( p = 0.027) and CD44v6 (p < 0.001). A loss of membranous staining and a progressive increase in cytoplasmic staining was observed from low to high grade SILs to ISCCs. Conclusions: Our study demonstrates that during the development of cervical lesions substantial qualitative ( subcellular localization-membrane to cytoplasmic) and quantitative alterations ( changes in expression) occur in the protein expression of E-cadherin, CD44, and CD44v6 in cervical cancer. The most striking observation was the decrease in membranous immunoreactivity and the progressive increase in cytoplasmic staining of E-cadherin, CD44 and CD44v6, relating to loss of differentiation as a consequence of neoplastic transformation. Copyright (C) 2004 S. Karger AG, Basel.