Assignment of SH3KBP1 to human chromosome band Xp22.1→p21.3 by in situ hybridization
Assignment of SH3KBP1 to human chromosome band Xp22.1→p21.3 by in situ hybridization
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DOI：
10.1159/000056966
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发表时间：
2001-01-01
期刊：
影响因子：
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通讯作者：
Taniyama, T
中科院分区：
文献类型：
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作者：
Narita, T;Amano, F;Taniyama, T
从人外周血中分离淋巴细胞，在添加10%胎牛血清和植物血凝素（PHA）的α-MEM中于37 ℃培养68-72小时。用BrdU处理淋巴细胞培养物以使细胞群同步。使用Gibco BRL BioNick标记试剂盒用dATP生物素化DNA探针，并进行FISH检测程序。结果表明，每个细胞中有1（3）个、2（70）个染色单体。
Lymphocytes isolated from human peripheral blood were cultured in α minimal essential medium (α-MEM) supplemented with 10% fetal bovine serum and phytohemagglutinin (PHA) at 37 C for 68-72 h. The lymphocyte cultures were treated with BrdU to synchronize the cell population. The DNA probe was biotinylated with dATP using the Gibco BRL BioNick labeling kit and procedure for FISH detection was performed. The results showed that the number of cells with specific signal were 1 (3), 2 (70), chromatids per cell.