USE OF A HEATED SUPERFICIAL HAND VEIN AS AN ALTERNATIVE SITE FOR THE MEASUREMENT OF AMINO-ACID-CONCENTRATIONS AND FOR THE STUDY OF GLUCOSE AND ALANINE KINETICS IN MAN
USE OF A HEATED SUPERFICIAL HAND VEIN AS AN ALTERNATIVE SITE FOR THE MEASUREMENT OF AMINO-ACID-CONCENTRATIONS AND FOR THE STUDY OF GLUCOSE AND ALANINE KINETICS IN MAN
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DOI：
10.1016/0026-0495(81)90074-3
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发表时间：
1981-01-01
影响因子：
9.8
通讯作者：
LACY, WW
中科院分区：
文献类型：
--
作者：
ABUMRAD, NN;RABIN, D;LACY, WW
比较了动脉和加热手浅静脉（HSHV）对人体氨基酸、乳酸、甘油、游离脂肪酸（FFA）、胰岛素和胰高血糖素的测量以及葡萄糖和丙氨酸动力学的测量。正常受试者（n = 8）在禁食过夜（12-14小时）后进行研究。u - 14c -丙氨酸和3,3h葡萄糖通过持续输注给予，并从放置在加热手（68度）的桡动脉和浅背静脉的导管中取样血液。（C环境），在对照期和通过持续输注l -氨基酸溶液实现的稳态高氨基酸血症期。在两个研究期间，测量的所有底物和激素的血液浓度以及冷和放射性葡萄糖和丙氨酸的浓度在2条血管中具有可比性。前臂深静脉（DV）的测量结果显示，与动脉或HSHV相比，血浆葡萄糖浓度较低（对照组为3%，实验期为5%），血浆丙氨酸浓度较高（对照组和实验期分别为13%和5%）。动脉或HSHV与DV之间的葡萄糖比活性差异轻微但不显著，而DV的血浆丙氨酸比活性明显低于动脉或HSHV（对照期32%，实验期14%），表明血液在前臂运输过程中发生了丙氨酸和葡萄糖的交换过程。因此，从DV中获得的血液样本会高估得出的全身葡萄糖和丙氨酸周转率。加热后的手浅静脉可以充分替代动脉测量全血氨基酸、乳酸、甘油以及血浆游离脂肪酸、胰岛素和胰高血糖素；它的使用可以避免与动脉导管插入术相关的风险，并且可以成为测量人体总葡萄糖和丙氨酸动力学的合适地点。
Comparisons were made between the artery and a heated superficial hand vein (HSHV) for the measurements of amino acids, lactate, glycerol, free fatty acids [FFA], insulin and glucagon and the measurements of glucose and alanine kinetics in man. Normal subjects (n = 8) were studied after an overnight fast (12-14 h). U-14C-alanine and 3, 3H glucose were administered by a constant infusion and blood was sampled from catheters placed in a radial artery and a superficial dorsal vein of a heated hand (68.degree. C environment), during a control period and a period of a steady-state hyperaminoacidemia achieved by a constant infusion of an L-amino acid solution. The blood concentrations of all substrates and hormones measured and the concentrations of cold and radioactive glucose and alanine were comparable in the 2 vessels during both study periods. Measurements obtained in a deep forearm vein (DV) showed the concentrations of plasma glucose to be lower (3% in the control period and 5% during the experimental period) and those of plasma alanine to be higher (13% and 5% during control and experimental periods, respectively) than the artery or the HSHV. The difference in glucose specific activity between the artery or the HSHV and the DV were slight but non-significant, while plasma alanine specific activity was significantly lower in the DV as compared to the artery or the HSHV (32% in the control period vs. 14% in the experimental period), suggesting a process of exchange of alanine and glucose occurring during the transit of blood across the forearm. As a result, blood samples obtained from a DV will overestimate the derived total body glucose and alanine turnover rates. The heated superficial hand vein can adequately replace the artery for the measurements of whole blood amino acids, lactate and glycerol and for plasma FFA, insulin and glucagon; its use can obviate the risks associated with arterial catheterization and can be a suitable site for the measurements of total body glucose and alanine kinetics in man.