EXPRESSION OF BASEMENT-MEMBRANE COMPONENTS THROUGH MORPHOLOGICAL-CHANGES IN THE HAIR-GROWTH CYCLE
EXPRESSION OF BASEMENT-MEMBRANE COMPONENTS THROUGH MORPHOLOGICAL-CHANGES IN THE HAIR-GROWTH CYCLE
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DOI：
10.1016/0012-1606(85)90033-8
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
GIBSON, WT
中科院分区：
文献类型：
--
作者：
COUCHMAN, JR;GIBSON, WT
与毛囊相关的纤维连接蛋白的数量和分布在大鼠毛发生长周期中发生变化。免疫细胞化学染色的毛囊中晚期生长期（生长阶段）揭示了存在的纤维连接蛋白的毛乳头基质中，在基底膜分离这从上皮细胞的毛球，并在基底膜和结缔组织鞘下的细胞外根鞘。在退化期早期，即过渡期，毛乳头基质染色消失。纤维连接蛋白存在于基底膜和结缔组织鞘中，退化期基底膜和结缔组织鞘增厚。卵泡缩短后，达到休止期（静止）阶段，此时发现纤连蛋白染色最小，仅限于次级胚芽周围的基底膜。生长期的开始，涉及细胞分裂和毛囊伸长，与该区域和毛乳头内及其周围的纤维连接蛋白的量的大量增加有关。通过[3H]胸苷掺入和放射自显影分析进入生长期，发现在纤维连接蛋白表达增加之前可以检测到生长。生长中的细胞，即使是在基底上的位置，在其表面也总是有一些纤连蛋白。早期生长期毛囊的免疫电镜证实了光镜下的发现，也表明，纤连蛋白存在于小泡接近毛乳头和一些上皮细胞的表面。层粘连蛋白和IV型胶原沉积增加，在早期生长期的卵泡，强调在体内形态发生事件的基底膜成分的重要性。
The amount and distribution of fibronectin associated with hair follicles was found to vary during the hair growth cycle in the rat. Immunocytochemical staining of follicles in mid-late anagen (the growth stage) revealed the presence of fibronectin in the dermal papilla matrix, in the basement membrane separating this from the epithelial cells of the hair bulb, and in the basement membrane and connective tissue sheath which underly the cells of the outer root sheath. Early in catagen, the transitional stage, staining of the dermal papilla matrix disappeared. Fibronectin persisted in the basement membrane and connective tissue sheath, which undergo corrugation and apparent thickening in catagen. After follicle shortening, the telogen (resting) stage is reached, at which point fibronectin staining was found to be minimal, being restricted to the basement membrane around the secondary germ. The onset of anagen, involving cell division and follicle elongation, was associated with a great increase in the amount of fibronectin in this zone and in and around the dermal papilla. Analysis of entry into anagen by [3H]thymidine incorporation and autoradiography revealed that growth could be detected before the increase in fibronectin expression. Growing cells, even in a suprabasal position, always had some fibronectin at their surface. Immunoelectron microscopy of early anagen follicles confirmed the light microscopic findings and also showed that fibronectin was present in small vesicles close to the surface of dermal papilla and some epithelial cells. Increased deposition of laminin and type IV collagen in early anagen follicles was noted, emphasizing the importance of basement membrane components during morphogenetic events in vivo.