Biological control of Fusarium wilt of tomato with hypovirulent binucleate Rhizoctonia in greenhouse conditions.
Biological control of Fusarium wilt of tomato with hypovirulent binucleate Rhizoctonia in greenhouse conditions.
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DOI：
10.1007/s10267-002-0084-x
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发表时间：
2003-04-01
期刊：
影响因子：
1.4
通讯作者：
Hyakumachi, Mitsuro
中科院分区：
文献类型：
--
作者：
Muslim, A.;Horinouchi, Hayato;Hyakumachi, Mitsuro
采用低毒力双核根核菌（HBNR）、G1、L2、W1和W7 4株菌株防治番茄枯萎病（FWT）。在单次和双次施用的5个试验中，HBNR分离株均能显著降低病害严重程度（叶面症状和茎内变色）。然而，HBNR分离株对疾病严重程度的降低取决于HBNR分离株和治疗方法。在4个分离株中，分离株HBNR W7能显著且持续地降低疾病严重程度（P=0.05）。施用HBNR菌株显著降低了番茄茎、根中FOL的菌落形成单位数（P=0.01）。菌株G1、W1和W7能显著提高植株（茎叶）鲜重（P=0.05）。这些结果表明，HBNR分离株有较大的潜力作为防治FWT的生物制剂。这是温室条件下利用HBNR生物防治FWT的首次报道。
Four isolates of hypovirulent binucleate Rhizoctonia (HBNR), G1, L2, W1, and W7 were used for control of Fusarium wilt of tomato (FWT) caused by Fusarium oxysporum f. sp. lycopersici (FOL). HBNR isolates could significantly reduce disease severity (foliar symptoms and discoloration inside the stem) among five experiments under single and double applications. Reduction of disease severity by HBNR isolates, however, differed depending on HBNR isolates and treatments. Of four isolates, isolate HBNR W7 could significantly and consistently reduce (P=0.05) disease severity. Application of HBNR isolates significantly reduced (P=0.01) the number of colony-forming units of FOL in stems and roots of tomato. Among the HBNR isolates, G1, W1, and W7 could significantly increase (P=0.05) fresh weight of the plants (stems and leaves). These results indicate that isolates of HBNR have a greater potential as biocontrol agents against FWT. This is the first report of biocontrol of FWT by HBNR under greenhouse conditions.