Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
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中国北疆细粒棘球绦虫的流行情况及分子特征
DOI：
10.3347/kjp.2019.57.2.153
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发表时间：
2019-04-01
影响因子：
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通讯作者：
Jia, Bin
中科院分区：
文献类型：
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作者：
Guo, Baoping;Zhang, Zhuangzhi;Jia, Bin
细粒棘球绦虫是一种重要的人畜共患寄生虫，在全球范围内引起人和动物的囊型棘球蚴病。本研究分析了大肠埃希菌CE的流行情况和cox 1基因序列的变异。新疆北方不同地区采集的细粒棘球绦虫。调查显示，3.5%的绵羊和4.1%的牛感染了CE。用PCR方法从所有阳性羊、牛中扩增出cox 1基因片段。此外，还纳入了4个地区的26份阳性样本。分离株均为E.严格意义上的粒细胞共包含15种单倍型（Hap 1 -15），聚为G1（90.1%，91/101）和G3（9.9%，10/101）2种基因型。Hap 1是最常见的单倍型（48.5%，49/101）。Hap 9在人类样品中被发现，表明绵羊和牛储存人类CE。表明E.细粒棘球绦虫可能影响该地区牲畜和人类的CE控制。
Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.