Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing.
Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing.
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DOI：
10.1038/nmat4782
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发表时间：
2017-03
期刊：
影响因子：
41.2
通讯作者：
Parker KK
中科院分区：
文献类型：
--
作者：
Lind JU;Busbee TA;Valentine AD;Pasqualini FS;Yuan H;Yadid M;Park SJ;Kotikian A;Nesmith AP;Campbell PH;Vlassak JJ;Lewis JA;Parker KK
几十年来，生物医学研究一直依赖于动物研究和传统细胞培养。最近，微生理系统(MPS)，也被称为芯片上的器官，在体外概括了自然组织的结构和功能，已经成为一种有前途的替代方案。然而，目前的MPS通常缺乏集成的传感器，并且它们的制造需要多步骤光刻工艺。在这里，我们介绍了一种通过多材料3D打印来制造新型仪器心脏微生理装置的简便方法。具体地说，我们设计了六种功能墨水，基于压阻、高电导和生物兼容的软材料，使软应变计传感器能够集成到微体系结构中，指导仿生层状心脏组织的自组装。我们验证了这些嵌入式传感器在细胞孵化器环境中提供了组织收缩应力的非侵入性电子读数。我们进一步应用这些设备来研究药物反应，以及四周内人类干细胞来源的板层心肌组织的收缩发育。
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multi-material 3D printing. Specifically, we designed six functional inks, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readout of tissue contractile stresses, inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell derived laminar cardiac tissues over four weeks.