"Candidatus Sonnebornia yantaiensis", a member of candidate division OD1, as intracellular bacteria of the ciliated protist Paramecium bursaria (Ciliophora, Oligohymenophorea)
"Candidatus Sonnebornia yantaiensis", a member of candidate division OD1, as intracellular bacteria of the ciliated protist Paramecium bursaria (Ciliophora, Oligohymenophorea)
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DOI：
10.1016/j.syapm.2013.08.007
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发表时间：
2014-02-01
影响因子：
3.4
通讯作者：
Warren, Alan
中科院分区：
文献类型：
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作者：
Gong, Jun;Qing, Yao;Warren, Alan
从烟台淡水池塘中分离到的绿草履虫（Parameciumbursaria）中发现了一株胞内细菌。该细菌数量丰富，仅存在于宿主细胞质中，与绿色小球藻一起沿着，形成了一个可以在纯水中存活至少一周的三伙伴联合体。细菌16 S rRNA基因的克隆、测序和系统发育分析表明，该细菌属于未培养的候选分裂OM，该分裂通常构成稀有生物圈的一部分。透射电子显微镜和荧光原位杂交（FISH）检测结果表明，该细菌通常位于内共生小球藻细胞的藻周膜附近，偶尔也会不规则地分布在宿主细胞质中。命名为烟台海桑（Sonnebornia yantaiensis），sp. nov.为新细菌。一个强有力的支持单系亚支，OD 1-p，其中包括新的物种，是公认的，这项研究强调，原生生物可以是稀有细菌类群的重要宿主。(C)2013年爱思唯尔有限公司。All rights reserved.
An intracellular bacterium was discovered in an isolate of Paramecium bursaria from a freshwater pond in Yantai, China. The bacteria were abundant and exclusively found in the cytoplasm of the host which, along with the green alga Chlorella, formed a three-partner consortium that could survive in pure water for at least one week. Cloning, sequencing and phylogenetic analysis of the bacterial 16S rRNA gene showed that the bacterium belonged to the uncultured candidate division OM, which usually forms part of the rare biosphere. Transmission electron microscopy and fluorescence in situ hybridization (FISH) with specific probes showed that the bacteria were usually located close to the perialgal membranes of endosymbiotic Chlorella cells, and occasionally irregularly distributed throughout the host cytoplasm. The name "Candidatus Sonnebornia yantaiensis" gen. nov., sp. nov. is proposed for the new bacterium. A strongly supported monophyletic subclade, OD1-p, which included the new species, was recognized and this study highlights that protists can be important hosts for rare bacterial taxa. (C) 2013 Elsevier GmbH. All rights reserved.